Applications and Products

How Archer NGS kits, controls and QC assays are being used.



Sep
12
2017

Archer® Analysis tool: CNV Visualization in v5.1

The CNV visualization tool within Archer Analysis was designed to maximize analytical capabilities for a variety of NGS library preparations. By giving the user the ability to customize the sensitivity settings based on assay and normalization requirements, copy number gains and losses, in addition to novel variants, can be detected with exon-level resolution and effortlessly tracked within the database.


Sep
8
2017

FLT3-ITD Detection Tech Note

ArcherDX scientists developed Archer® VariantPlex® myeloid assays to detect FLT3-ITDs, along with other myeloid-specific variants, by targeted next-generation sequencing (NGS). A new technical note discusses how Anchored Multiplex PCR (AMP™) chemistry in conjunction with the data-optimized analysis algorithm in Archer Analysis enable accurate detection of FLT3-ITDs across all sizes and insertion sites from clinical-type samples with 100% accuracy.


Aug
14
2017

AMPlify your immune repertoire sequencing

AMP enrichment chemistry and Archer Analysis software reduce PCR bias and minimize PCR and sequencing errors that plague amplicon-based approaches by delivering reliable BCR and TCR sequencing. Immunoverse TCR and BCR kits enable quantitative and sensitive clonotype detection, with sequences detectable at fractions as low as one in a million. Coupled with the fully automated Archer Analysis software and user-owned data, a wide variety of research applications are available to you.


Apr
12
2016

Comprehensive Thyroid and Lung (CTL) kits enable parallel detection of copy number variants and expression levels by next-generation sequencing

CNVs are common oncogenic drivers in lung cancer that result in altered expression profiles. Here, we show that AMP™ enables sensitive NGS-based detection of CNVs and expression profiling of multiple genes relevant to lung cancer.


Apr
6
2016

Anchored Multiplex PCR enables sensitive detection and internal verification of novel gene fusions

Current methods to detect gene fusions in clinical samples, such as FISH and IHC, cannot identify novel fusion partners. AMP-based NGS is a sensitive method to detect both known and unknown gene fusions. In this article, we show that AMP-based NGS detected an EML4-ALK fusion in a sample that was ambiguous by ALK break-apart FISH testing.


Apr
5
2016

Breakpoint identification is more sensitive than expression imbalance to detect gene fusions

Expression imbalance can be used to infer the presence of a gene fusion. However, recent data suggest that breakpoint identification may be a more accurate approach. Here, we show that breakpoint identification is more sensitive than expression imbalance to detect gene fusions by AMP-based NGS.


Feb
10
2016

ArcherDX target enrichment kits for NGS detect all major types of oncogenic MET aberrations

Deregulation of the receptor tyrosine kinase, MET, is implicated in a variety of human cancers. This article reviews the underlying genetic aberrations that drive MET deregulation, and how each of these can be detected with target enrichment kits provided by ArcherDX, Inc. for use in downstream NGS assays.


Feb
5
2016

Learn How DNA Integrity Affects Detection Sensitivity and What You Can Do to Maximize Sensitivity

FFPE storage of clinical specimens damages DNA, resulting in poor sequencing coverage and lowered sensitivity of variant detection. Read on to find out how to overcome this issue using the Archer PreSeq DNA QC Assay and online genome calculator.


Jan
20
2016

Next-Generation Sequencing is More Accurate Than Fluorescence in Situ Hybridization to Detect Malignancy in Cytologic Specimens

What benefits does addition of NGS or FISH impart on assessment of neoplastic pancreaticobiliary malignancies? This study provides some exciting answers.


Dec
10
2015

Technical Note: Archer Analysis Variant and CNV detection methods

Technical note describing variant and CNV detection methods for Archer Analysis software version 4.0.0.


Dec
10
2015

Technical Note: The use of molecular barcodes in Anchored Multiplex PCR

The advantages of molecular barcodes (MBCs) with Anchored Multiplex PCR chemistry.


Dec
10
2015

Technical Note: Beyond Nanograms - Amplifiable genomes to assess NGS library complexity from FFPE samples

Introduction of the PreSeq DNA QC Assay, a simples qPCR assay that facilitates the quantification of the number of sequenceable copies of input genomic DNA, thereby maximizing information recovery from NGS libraries.


Dec
1
2015

Technical Note: Comprehensive report on FFPE Extraction Methods

Recommendations for extraction kit-specific conditions for nucleic acid preparation from FFPE that are compatible with FusionPlex and VariantPlex library preparation.


Nov
13
2015

AMP 2015 Poster

Presented results from a NSCLC screen using a pair of targeted NGS panels that permit detection of SNVs, indels, CNVs and rearrangements across oncogenes and tumor suppressors relevant to solid tumors from the 2015 Annual Molecular Pathology Meeting.


Nov
4
2015

Technical Note: PreSeq™ RNA QC Assay predicts high-confidence libraries

PreSeq Cq values exhibit a strong concordance with the number of mean control RNA start sites in a library, and correspondingly, a strong agreement with Archer Analysis QC pass.


Sep
15
2015

Can you trust your gene fusion-negative results?

Orthogonal verification of fusion-negative FFPE samples using Archer FusionPlex assays revealed a rare MEC-specific fusion (CRTC3-MAML2) in NSCLC


Jul
22
2015

RNA Fusion Sensitivity Analysis

Here we describe a sensitivity experiment we conducted with a custom panel we designed for the detection of gene fusions in the ALK, BDNF, NGF, NGFR, NTF3, NTRK1,2 and 3, RET and ROS1 genes that are often found to be involved in various lung cancers.


Feb
25
2015

CNV Detection Poster

Development and validation of a directed, rapid and highly multiplexed assay to detect copy number variations in clinical samples - Poster, AGBT 2015


Feb
25
2015

FusionPlex NGS Analysis Poster

Archer™ FusionPlex™ NGS Data Analysis, Algorithms and Methods: Hypothesis-Free Gene Fusion Detection - Poster from AGBT 2015 Conference


Feb
25
2015

VariantPlex BRCA Panel Poster

Optimization of nested PCR conditions to improve target coverage uniformity in Archer™ VariantPlex™ BRCA Panel - Poster, AGBT 2015


Nov
21
2013

AMP for Targeted NGS Poster

Anchored Multiplex PCR for Targeted Next Generation Sequencing - Poster, AMP 2013

How to contact us

Address

2477 55th Street, Suite 202

Boulder, CO 80301

Phone

Phone: (877) 771 1093

Phone: (303) 357 9001

All content © 2017 ArcherDX, Inc.

For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.