Internal tandem duplications (ITDs) in the FLT3 gene are detected in about 25% of acute myeloid leukemia (AML) samples and are associated with a poor prognosis. Furthermore, FLT3-ITD expressed kinases are sensitive to tyrosine kinase inhibitors, making them a target of interest for the development of novel AML treatments. However, detecting FLT3-ITDs from NGS data, alongside other mutation types common in AML, can be challenging.
ArcherDX scientists developed Archer® VariantPlex® myeloid assays to detect FLT3-ITDs, along with other myeloid-specific variants, by targeted next-generation sequencing (NGS). A new technical note discusses how Anchored Multiplex PCR (AMP™) chemistry in conjunction with the data-optimized analysis algorithm in Archer Analysis enable accurate detection of FLT3-ITDs across all sizes and insertion sites from clinical-type samples. The table below shows that FLT3-ITDs were detected in AML-positive blood and bone marrow samples with 100% accuracy using these VariantPlex assays.
Download the tech note for more details and data.FLT3-ITD AMP tech note
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