The Archer® FusionPlex® ALL Kit is a targeted next-generation sequencing assay that detects and identifies fusions, point mutations and expression levels from RNA input. The kit is comprehensive in nature, targeting 81 genes associated with Acute Lymphoblastic Leukemia, including all recurrent Ph-Like ALL fusion events. Using Archer’s proprietary Anchored Multiplex PCR™-based enrichment, fusions of all genes in this kit can be identified in a single sequencing assay, even without prior knowledge of fusion partners or breakpoints. Additionally, select genes are targeted for molecular barcode-enabled relative expression level detection and point mutation coverage.
For Research Use Only. Not for use in diagnostic procedures.
AB0013, AB0014Product Insert - FusionPlex® ALL
Note: Fusions involving BCR and TCR loci, including IGH, IGL and IGK, are targeted for expression and may not be explicitly called as a fusion because these often do not result in chimeric transcripts.
|Input RNA required||50-200ng|
|Time required||8.5 hours|
|Hands-on time||3.5 hours|
|Sample type(s)||Blood, fresh frozen, FFPE|
|# of GSP2s||506|
|Unique moleculer on-target||>95%|
|Coverage uniformity > 20% mean||>99%|
|% Bases at 50x unique molecule coverage||>99%|
|Platform||Illumina® or Ion Torrent™|
|Recommended # of reads||1.5 Million|
Note: As observed on multiple lots using 50ng gDNA input. DNA input allows for a better measurement of the behavior across primers and can be be used to assess coverage uniformity. RNA can vary due to expression levels.
The FusionPlex ALL kit includes targets found in Philadelphia chromosome-like acute lymphoblastic leukemia (Ph-like ALL), which is characterized by modifications in lymphoid transcription factor genes, poor outcomes, and a gene-expression profile similar to that of BCR-ABL1 ALL. Recent clinical and xenograft studies have revealed that the Ph-like ALL gene expression profile includes multiple genomic alterations, which are listed in the table below.
|Kinase||Number of gene partners||Fusion partner genes|
|ABL1||7||ETV6, NUP214, RCSD1, RANBP2, SNX2, ZMIZ1|
|ABL2||3||PAG1, RCSD1, ZC3HAV1|
|PDGFRB||4||EBF1, SSBP2, TNIP1, ZEB2|
|JAK2||10||ATF7IP, BCR, EBF1, ETV6, PAX5, PPFIBP1, SSBP2, STRN3, TERF2, TPR|
*FusionPlex ALL Kit will only detect an EPOR truncation
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