Introduction

The Archer® FusionPlex® Lymphoma Kit is a targeted next generation sequencing assay to simultaneously detect and identify fusions, point mutations, and expression levels found in RNA transcripts in 125 genes that are linked to lymphomas. Using Anchored Multiplex PCR™-based enrichment, fusions can be identified even without prior knowledge of fusion partners or breakpoints. Additionally, select genes are targeted for molecular barcode-enabled relative expression detection and coverage over key hotspot regions.

For Research Use Only. Not for use in diagnostic procedures.

Highlights

  • Detect known and novel fusions associated with lymphomas
  • Relevant point mutations are built into the kit to maximize the information obtained from your RNA sample
  • Molecular barcode-enabled relative expression detection in genes relevant for GCB vs. ABC DLBCL distinction
  • Wide sample type compatibility, including FFPE
  • Built-in expression detection for immune checkpoint markers


FusionPlex Lymphoma Kit Targets

  •  Fusion, splicing or exon skipping
  •  Mutation
  •  Expression
AICDA
   
CCND2
   
DNMT3A
   
JAK1
   
NEK6
   
RAB29
   
AKT3
   
CCND3
  
DNMT3B
   
JAK2
  
NFKB1
   
RAG1
   
ALK
  
CD274
   
DNTT
   
JAK3
   
NFKB2
   
RAG2
   
ASB13
   
CD44
   
DUSP22
   
KIAA0101
   
NME1
   
RANBP1
   
BATF3
   
CD79B
   
E2F2
   
KMT2A
   
NOTCH1
  
RHOA
   
BAX
   
CDC25A
   
EIF4A1
   
KRAS
   
NOTCH2
   
S1PR2
   
BCL2
  
CDK6
   
ENTPD1
   
LIMD1
   
NRAS
   
SERPINA9
   
BCL2A1
   
CDKN2A
   
ETV6
  
LMO2
   
P2RY8
   
SF3B1
   
BCL3
  
CDKN2B
   
EXOC2
   
LRMP
   
PAICS
   
SH3BP5
   
BCL6
  
CEBPD
   
EZH2
   
LZTS1
   
PDCD1
   
STAT3
   
BCR
   
CEBPE
   
FAM216A
   
MAL
   
PDCD1LG2
  
STAT5B
   
BIRC3
  
CEBPG
   
FBXW7
   
MALT1
   
PDGFRA
  
STAT6
   
BLNK
   
CHIC2
   
FGFR1
   
MAML3
   
PIM1
   
STIL
   
BMF
   
CIITA
   
FOXP1
   
MKL1
   
PIM2
   
STRBP
   
BMP7
   
CREB3L2
   
FUT8
   
MLF1
   
PLCG1
   
TCF3
   
BRAF
   
CREBBP
  
IDH1
   
MLLT10
   
PLCG2
   
TNFRSF13B
   
BTK
   
CTLA4
   
IDH2
   
MME
   
PPAT
   
TNFSF4
   
CARD11
   
CYB5R2
   
IL16
   
MUC1
   
PRDM16
   
TP63
   
CBFB
   
DEK
   
IRF4
   
MYBL1
   
PRKAR2B
   
WT1
  
CCDC50
   
DENND3
   
IRF8
   
MYC
  
PTPN1
   
XPO1
   
CCND1
 
DLEU1
   
ITPKB
   
MYD88
   
PYCR1
   

Notes

  • Fusions involving BCR and TCR loci, including IGH, IGL and IGK, are targeted for expression and may not be explicitly called as a fusion because these often do not result in chimeric transcripts.
  • Targets are listed for primary purpose but may detect other mutation types.
  • ABC and GCB DLBCL signature genes are targeted for expression. An experimental classification tool for ABC/GCB differentiation is available in Archer Analysis.

FusionPlex Lymphoma Kit superior panel performance


Feature Specification
Input RNA required 50-200ng
Time required 8.5 hours
Hands on time 3.5 hours
Sample type Blood, fresh frozen, FFPE
# of GSP2s 716
Unique molecule on-target >90%
Coverage uniformity >20% mean >99%
% Bases at 50x unique molecule coverage >99%
Platform Illumina® or Ion Torrent™
Recommended # of reads 2 million

*As observed on multiple lots using 50ng gDNA input. DNA input allows for a better measure of the behavior across primers, and can be be used to assess coverage uniformity. RNA can vary by due to expression levels.

How to contact us

Address

2477 55th Street, Suite 202

Boulder, CO 80301

Phone

Phone: (877) 771 1093

Phone: (303) 357 9001

All content © 2017 ArcherDX, Inc.

For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.