Introduction

The Archer® FusionPlex® Pan-Heme Kit is an Anchored MultiPlex PCR™-based next-generation sequencing assay that detects and identifies fusions, point mutations and expression levels from RNA input. The kit encompasses all the targets in the FusionPlex heme kits, amounting to over 199 genes relating to lymphoid and myeloid malignancies. By using gene-specific primers to amplify into molecular barcodes ligated onto the cDNA fragment ends, both known and novel fusions can be identified. Additionally, unique molecule identification enables expression detection in select genes.

For Research Use Only. Not for use in diagnostic procedures.

Highlights

  • Comprehensive driver mutation detection for leukemia and lymphoma – including known and novel fusion events
  • Targets key hotspots regions for simultaneous point mutation detection
  • Molecular barcode-enabled expression detection for key genes including immune checkpoint and DLBCL subtyping
  • Expression imbalance enabled in select genes for secondary fusion confirmation


FusionPlex Pan-Heme Kit Targets

  •  Fusion, splicing or exon skipping
  •  Mutation
  •  Expression
  •  Expression Imbalance
ABL1
 
CDKN2A
   
FAM216A
   
KRAS
   
NUP98
   
RUNX1T1
  
ABL2
   
CDKN2B
   
FBXW7
   
LIMD1
   
P2RY8
   
S1PR2
   
AICDA
   
CEBPA
  
FGFR1
   
LMO1
   
PAG1
   
SEMA6A
   
AKT3
   
CEBPD
   
FGFR2
   
LMO2
   
PAICS
   
SERPINA9
   
ALK
  
CEBPE
   
FGFR3
   
LRMP
   
PAX5
  
SETBP1
   
>ASB13
   
CEBPG
  
FLT3
  
LYL1
   
PBX1
  
SETD2
   
ASXL1
   
CHD1
   
FOXP1
   
LZTS1
   
PDCD1
   
SF3B1
   
BATF3
   
CHIC2
   
FUT8
   
MAL
   
PDCD1LG2
  
SH2B3
   
BAX
   
CIITA
   
GATA1
   
MALT1
   
PDGFRA
  
SH3BP5
   
BCL11B
   
CREB3L2
   
GATA2
   
MAML3
   
PDGFRB
   
SLC29A1
   
BCL2
 
CREBBP
  
GLIS2
   
MECOM
  
PHF6
   
SOX11
   
BCL2A1
   
CRLF2
 
GNAS
   
MKL1
   
PICALM
   
SRSF2
   
BCL3
  
CSF1R
   
HOXA10
   
MLF1
   
PIM1
   
STAT3
   
BCL6
  
CSF3R
   
HOXA9
   
MLLT10
   
PIM2
   
STAT5B
   
BCR
   
CTLA4
   
ID4
   
MLLT4
   
PLCG1
   
STAT6
   
BIRC3
  
CYB5R2
   
IDH1
   
MME
   
PLCG2
   
STIL
   
BLNK
   
DCK
   
IDH2
   
MPL
   
PML
  
STRBP
   
BMF
   
DEK
   
IKZF1
   
MUC1
   
PPAT
   
TAL1
  
BMP7
   
DENND3
   
IKZF2
   
MYBL1
   
PRDM16
   
TCF3
   
BRAF
   
DLEU1
   
IKZF3
  
MYC
  
PRKAR2B
   
TFG
   
BTK
   
DNM2
   
IL16
   
MYD88
  
PTK2B
   
TLX1
   
CALR
   
DNMT3A
   
IL7R
   
MYH11
  
PTPN1
   
TLX3
   
CARD11
   
DNMT3B
   
IRF4
   
NEK6
   
PTPN11
   
TNFRSF13B
   
CBFB
   
DNTT
   
IRF8
   
NF1
   
PYCR1
   
TNFSF4
   
CBL
   
DUSP22
   
ITPKB
   
NFKB1
   
P2PR8
   
TP63
   
CCDC50
   
E2F2
   
JAK1
   
NFKB2
   
RAB29
   
TYK2
   
CCND1
 
EBF1
   
JAK2
  
NME1
   
RAG1
   
U2AF1
   
CCND2
   
EIF4A1
   
JAK3
   
NOTCH1
  
RAG2
   
WT1
  
CCND3
  
ENTPD1
   
KAT6A
   
NOTCH2
   
RANBP1
   
XPO1
   
CD274
   
EPOR
   
KDM6A
   
NPM1
   
RARA
 
ZCCHC7
   
CD44
   
ERG
   
KIAA0101
   
NRAS
   
RBM15
   
CD79B
   
ETV6
  
KIT
   
NT5C2
   
RHOA
   
CDC25A
   
EXOC2
   
KLF2
   
NTRK3
  
ROS1
  
CDK6
   
EZH2
   
KMT2A
   
NUP214
   
RUNX1
  

Notes

  • Fusions involving BCR and TCR loci, including IGH, IGL and IGK, are targeted for expression and may not be explicitly called as a fusion because these often do not result in chimeric transcripts.
  • Targets are listed for primary purpose but may detect other mutation types.
  • ABC and GCB DLBCL signature genes are targeted for expression. An experimental classification tool for ABC/GCB differentiation is available in Archer Analysis.

FusionPlex Pan-Heme superior kit performance

50-200ng

Input RNA required

9.5 hours

Time required

2.5 hours

Hands on time

1,054

# of GSP2s

>99%

% Bases at 50x unique molecule coverage

>90%

Unique molecule on-target

>98%

Coverage uniformity >20% mean

4.5 million

Recommended # of reads

Illumina® or Ion Torrent™

Platform

Blood, fresh frozen, FFPE

Sample type

As observed on multiple lots using 50ng gDNA input. DNA input allows for a better measure of the behavior across primers, and can be be used to assess coverage uniformity. RNA can vary by due to expression levels.

How to contact us

Address

2477 55th Street, Suite 202

Boulder, CO 80301

Phone

Phone: (877) 771 1093

Phone: (303) 357 9001

All content © 2017 ArcherDX, Inc.

For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.