Introduction

The Archer® FusionPlex® Myeloid Kit is a targeted next-generation sequencing assay that detects and identifies fusions, point mutations and expression levels from RNA input. The kit is comprehensive in nature, targeting 84 genes associated with myeloid-origin malignancies. Using Archer’s proprietary Anchored Multiplex PCR™-based enrichment, fusions of all genes in this kit can be identified in a single sequencing assay, even without prior knowledge of fusion partners or breakpoints. Additionally, select genes are targeted for molecular barcode-enabled relative expression level detection and point mutation coverage.

For Research Use Only. Not for use in diagnostic procedures.

Highlights

  • Simultaneously interrogate hundreds of potential gene fusion events and relevant point mutations
  • Molecular barcode-enabled expression level detection in relevant genes
  • Expression imbalance enabled in select genes for secondary fusion confirmation
  • Highly multiplexed, requiring less than 2 million reads

Kit Targets

FusionPlex Myeloid Kit targets

  •  Fusion, splicing or exon skipping
  •  Mutation
  •  Expression
  •  Expression Imbalance
ABL1
 
DNM2
   
IKZF1
   
MUC1
   
PTPN11
   
AKT3
   
DNMT3A
   
IKZF3
  
MYC
  
RARA
 
ASXL1
   
ERG
   
IRF4
   
MYD88
   
RBM15
   
BCR
   
ETV6
  
IRF8
   
MYH11
  
ROS1
  
BRAF
   
EZH2
   
JAK1
   
NF1
   
RUNX1
  
CALR
   
FBXW7
   
JAK2
  
NOTCH1
  
RUNX1T1
  
CBFB
   
FGFR1
   
JAK3
   
NPM1
   
SETBP1
   
CBL
   
FGFR2
   
KAT6A
   
NRAS
   
SETD2
   
CD274
   
FGFR3
   
KDM6A
   
NUP214
   
SF3B1
   
CEBPA
  
FLT3
  
KIT
   
NUP98
   
SLC29A1
   
CHD1
   
GATA1
   
KMT2A
   
PDCD1
   
SRSF2
   
CHIC2
   
GATA2
   
KRAS
   
PDCD1LG2
  
TCF3
   
CREBBP
  
GLIS2
   
MECOM
  
PDGFRA
  
TFG
   
CSF1R
   
GNAS
   
MKL1
   
PDGFRB
   
U2AF1
   
CSF3R
   
ID4
   
MLLT10
   
PHF6
   
WT1
  
CTLA4
   
IDH1
   
MLLT4
   
PICALM
   
XPO1
   
DCK
   
IDH2
   
MPL
   
PML
  



Superior kit performance

50-200ng

Input RNA required

9.5 hours

Time required

2.5 hours

Hands-on time

507

# of GSP2s

>99%

% Bases at 50x unique molecule coverage

>96%

Unique molecule on-target

>99%

Coverage uniformity > 20% mean

1.5 Million

Recommended # of reads

Illumina® or Ion Torrent™

Platform

Blood, fresh frozen, FFPE

Sample type

As observed on multiple lots using 50ng gDNA input. DNA input allows for a better measure of the behavior across primers, and can be be used to assess coverage uniformity. RNA can vary by due to expression levels.



Open-ended translocation identification


While break-apart FISH can detect both known and novel fusions, sequence information is not obtained and thus FISH cannot identify fusion partner genes nor can it detect other mutations, such as resistance mutations, within fusion genes. Single, unidirectional gene-specific primers (GSPs) used for AMP enable open-ended amplification of fusion genes, identifying fusion breakpoints and partners genes at nucleotide resolution. For example, AMP enabled NGS-based detection of a KMT2A-MLLT3 fusion from reads originating from KMT2A GSPs. Here, these unidirectional KMT2A GSPs enabled amplification and sequencing through the fusion breakpoint between exon 9 of KMT2A and exon 11 of MLLT3, and extended 6 exons into MLLT3.


What translocation is this?


How to contact us

Address

2477 55th Street, Suite 202

Boulder, CO 80301

Phone

Phone: (877) 771 1093

Phone: (303) 357 9001

All content © 2017 ArcherDX, Inc.

For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.