Immune repertoire sequencing assays

Repertoire analysis on your own terms

Archer® Immunoverse™ kits are targeted NGS assays to characterize the human immune repertoire from RNA input. Powered by Anchored Multiplex PCR (AMP™), the lyophilized kits uniquely tag and amplify V(D)J rearrangements for sequencing on Illumina® platforms. Sequenced libraries are analyzed using the Immune Repertoire pipeline in Archer Analysis—a powerful and transparent tool for clonotype identification and frequency reporting.

Product Information

Illumina® Protocol
Demo Data





Anchored Multiplex PCR (AMP™)-powered V(D)J amplification

The Immunoverse assays utilize AMP chemistry for open-ended amplification from molecular barcoded (MBC) adapters, eliminating the need for complicated opposing primer-based amplification schemes and uniformity assumptions. This unbiased approach towards VDJ recombination can yield more than 100,000 clonotypes in a single reaction, without the fear of PCR bias. The figure above shows a simplified schematic of AMP-enabled V(D)J enrichment.

Archer molecular barcode adapters enable amplification using a single gene-specific primer amplifying against a ligated adapter.
Schematic of Archer's Molecular Barcode Adapters (MBCs).

Molecular barcode-based deduplication and error correction enable true quantification

Archer Analysis utilizes the AMP-specific molecular barcode adapters for deduplication and PCR sequencing error correction to provide a true measure of sample complexity. Additionally, unique molecule identification enables highly quantitative analysis of clonotype frequencies.

Archer molecular barcode adapters enable identification of unique starting molecules, de-duplication and error-correction.


Single-use lyophilized library prep reagents

  • Break-away, reactions and bring forward any amount of reactions
  • Lyophilized reagents minimize user error and contamination
  • Simple workflow requiring less than 3.5 hours hands-on-time and 8.5 hours total

Flexible input requirements

  • Flexible input requirements, from 25ng to 2µg of RNA from PMBC, FFPE, or Fresh frozen tissue
Archer Immunoverse assay workflow overview


Sensitive clonotype identification

Jurkat cell line dilution in PBL background down to 10-6 from 400ng input shows strong linearity and detection of jurkat sequences at expected frequencies across replicates

Libraries were prepped using the Immunoverse TCR beta/gamma kit and sequenced on an Illumina® NextSeq® using a 300-cycle kit. Libraries were normalized to 0.5M reads and 2.5M reads (dilution 6) and analyzed with Archer Analysis.



Precise quantitation across a broad dynamic range

Highly reproducible clonotype frequency reporting of top 10 clones between replicates in jurkat dilution into PBL background

Libraries were prepped from 400ng input using the Immunoverse TCR beta/gamma kit and sequenced on an Illumina® MiSeq® using a 600-cycle kit. Libraries were normalized to 0.6M reads and analyzed with Archer Analysis.



Consistent performance between labs

Strong linearity and reproducibility is shown in dilution series across two different laboratories

Ten-fold serial dilutions of Jurkat cell line mRNA into PBL background we performed in duplicates in two labs. Libraries were sequenced on Illumina® NextSeq® with 2x150bp reads and normalized to 0.5M reads and analyzed using archer analysis.



Repertoire analysis on your own terms

Archer Analysis' point-and-click interface dynamically visualizes your clonotype information.

Archer Analysis, a powerful and transparent tool for immune repertoire analysis, providing extensive clone and clonotype information in custom-filtered tables, dynamic visualizations, and full data exports to answer advanced research questions. Archer analysis is available for private cloud and local installation behind your firewall.






Get started with Immunoverse Assays


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Demo data and training available upon request




How to contact us

Address

2477 55th Street, Suite 202

Boulder, CO 80301

Phone

Phone: (877) 771 1093

Phone: (303) 357 9001

All content © 2017 ArcherDX, Inc.

For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.