Tech Notes

  •  Archer Analysis Variant and CNV detection methods

    This document describes the DNA software applications in Archer Analysis version 4.0.0

  •  Comprehensive Report on FFPE Extraction Methods

    Six popular commercially-available FFPE extraction kits are tested for performance in conjunction with Anchored Multiplex PCR (AMP™) chemistry.

  •  Detection of Internal Tandem Duplications in FLT3 with Anchored Multiplex PCR and Next-Generation Sequencing

    FLT3 encodes a receptor tyrosine kinase that is involved in p53 activation and has roles in cell growth arrest and apoptosis. Internal tandem duplications (ITDs) in the juxtamembrane domain result in constitutive activation of FLT3, causing aberrant cell growth leading to tumorigenesis. FLT3-ITDs are associated with poor prognosis of acute myeloid leukemia (AML) and are detected in about 25% of AML cases. As FLT3-ITD expressed kinases are sensitive to tyrosine kinase inhibitors, they are of considerable interest for the development of novel AML treatments.

  •  PreSeq DNA QC Assay - Beyond Nanograms: Amplifiable Genomes to assess NGS library complexity from FFPE samples

    The PreSeq DNA QC Assay is a simple qPCR assay that facilitates the quantification of sequenceable copies of input genomic DNA, thereby maximizing information recovery from NGS libraries.

  •  PreSeq RNA QC Assay predicts high-confidence libraries

    RNA derived from formalin-fixed, paraffin-embedded (FFPE) tissue can be of variable quality due to chemical modification incurred during the fixation process as well as during pre-fixation handling. While many different methods are available for measuring RNA mass and fragment length, we have developed the Archer™ PreSeq™ RNA QC Assay, a qPCR-based transcript analysis assay that is highly predictive of sequencing success using Archer FusionPlex™ Assays.

  •  The Use of Molecular Barcodes in Anchored Multiplex PCR

    Sample barcoding, also called sample indexing, is a common approach to labeling samples for multiplex sequencing and analysis. All nucleic acids in a sample are labeled with the same sequence tag, and the result- ing library is pooled with other libraries and sequenced in parallel in a single run. Then, during analysis, the sample-speci c indexes enable the software to sepa- rate the multiplexed sequence data in sample-speci c data sets

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For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.