This document describes the DNA software applications in Archer Analysis version 4.0.0
Six popular commercially-available FFPE extraction kits are tested for performance in conjunction with Anchored Multiplex PCR (AMP™) chemistry.
FLT3 encodes a receptor tyrosine kinase that is involved in p53 activation and has roles in cell growth arrest and apoptosis. Internal tandem duplications (ITDs) in the juxtamembrane domain result in constitutive activation of FLT3, causing aberrant cell growth leading to tumorigenesis. FLT3-ITDs are associated with poor prognosis of acute myeloid leukemia (AML) and are detected in about 25% of AML cases. As FLT3-ITD expressed kinases are sensitive to tyrosine kinase inhibitors, they are of considerable interest for the development of novel AML treatments.
The PreSeq DNA QC Assay is a simple qPCR assay that facilitates the quantification of sequenceable copies of input genomic DNA, thereby maximizing information recovery from NGS libraries.
RNA derived from formalin-fixed, paraffin-embedded (FFPE) tissue can be of variable quality due to chemical modification incurred during the fixation process as well as during pre-fixation handling. While many different methods are available for measuring RNA mass and fragment length, we have developed the Archer™ PreSeq™ RNA QC Assay, a qPCR-based transcript analysis assay that is highly predictive of sequencing success using Archer FusionPlex™ Assays.
Sample barcoding, also called sample indexing, is a common approach to labeling samples for multiplex sequencing and analysis. All nucleic acids in a sample are labeled with the same sequence tag, and the result- ing library is pooled with other libraries and sequenced in parallel in a single run. Then, during analysis, the sample-speci c indexes enable the software to sepa- rate the multiplexed sequence data in sample-speci c data sets
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