What extraction methods are supported for my input material prior to library preparation with the Archer FusionPlex and VariantPlex assays?

For all extraction methods below we recommend the following elution buffer and methods for quantification and QC:

Recommended Elution Buffer Quantification Method Recommended Quality Check Step
nuclease-free water
  • Qubit® RNA HS assay kit (Life Technologies® Q32852) for FusionPlex
  • Qubit® HS dsDNA (Life Technologies® Q32851) for VariantPlex
  • Archer® PreSeq RNA QC Assay (AK0043-16) for FusionPlex®
  • Archer® PreSeq DNA QC Assay (AK0067-24) for VariantPlex

Total Nucleic Acid Extraction from Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue

Recommended Extraction Method Extraction Kit Protocol Recommendations
Agencourt® FormaPure® Total Nucleic Acid Extraction (A33342)
  • DO NOT treat with DNase.
  • Use heat blocks versus water baths.
  • Perform a one-hour digestion at 55°C at Step 5. Do not digest overnight.
  • Perform reverse-crosslinking by incubating one hour at 80°C between Steps 5 and 6.
  • Elute the sample in 40┬ÁL nuclease-free water at Step 23.
Promega ReliaPrep FFPE Total RNA Miniprep System (Z1001)
  • Extend the reverse crosslinking step at 80°C to one hour.
Maxwell RSC RNA FFPE Kit (AS1440)
  • Do not DNase treat. Proceed directly from 15 minutes at room temperature to centrifugation at full speed for 2 minutes.

Fresh-Frozen Tissue (FF), Cell Lines and Blood

Recommended Extraction Method
Any total RNA (for FusionPlex) or DNA (for VariantPlex) extraction kit


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For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.