How do I set the ramp rate on my thermal cycler?

Refer to the instructions for use specific to your instrument. Please see table below for details regarding ramp rates for some common thermal cyclers.

CompanyThermal Cycler ModelBlock TypeMaximum Block Ramp Rate (°/s)Notes
Applied Biosystems® GeneAmp® PCR System 9700 60 wells 1.9
96-Aluminum 2.3
96-Silver/Gold 2.6
2 x 96 1.6
3 x 384 1.8
Life Technologies™ Veriti® Standard 96 3.9
Fast 96 5.0
60 wells 3.3
Bio-Rad® T100™ 96 wells 4.0 Set ramp % under the denaturing step of the amplification cycle; can only be set at 0.2°C or 0.3°C/s. Default to 0.2°C/s in this case
C1000™ 96 wells; 96 wells deep block; 2 x 48 block; 384 well block 5.0
S1000™ 96 wells; 96 wells deep block; 384 well block 5.0
Eppendorf® Mastercycler® Pro 96-Aluminum 4.0/3.0 Block ramp rate – (heating ramp rate/cooling ramp rate)
Mastercycler Pro 5 96-Silver 6.0/4.5 Block ramp rate – (heating ramp rate/cooling ramp rate)
Mastercycler Pro 384 Aluminum 4.0/3.0 Block ramp rate – (heating ramp rate/cooling ramp rate)


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For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.