The modified ramp rate in the v2 kit is vital to the assays' function due to the complex nature of the Anchored Multiplexed PCR reaction. It is important to verify that this ramp rate is set correctly before starting the PCR program by consulting the instrument’s user manual or contacting the manufacturer. Please see "How do I set the ramp rate on my thermal cycler?" for information on ramp rates for specific thermal cyclers.
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