Why is my final Archer library concentration low?

The source of the nucleic acid, the purification method, the chemical the nucleic acid is suspended in and the starting amount of nucleic acid can contribute to low library concentration. Recommendations for improving library yield include:

  • Extract TNA from FFPE tissue with one of our recommended methods.
  • Make sure input quantity was measured using the appropriate Qubit® assay.
  • Perform the Archer PreSeq QC assay to determine quality.

Incorrect PCR cycling can also lead to low library yields. Ensure that the cycling temperatures, cycle numbers and ramp rates are accurate. Check the instrument to make sure it is calibrated and in good working order.


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For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.