What values should I expect for cluster density on Illumina® sequencing runs?

The expected cluster density will vary by instrument type and kit version. Please see table below for details.

Instrument Kit Expected Cluster Density
HiSeq® High Output, TruSeq v3 750-850 k/mm2
High Output, TruSeq v4 950-1050 k/mm2
Rapid, v2 850-1000 k/mm2
MiSeq® v2 1000-1200 k/mm2
v3 1200-1400 k/mm2
MiniSeq® Mid & High Output 170-220 k/mm2
NextSeq® Mid & High Output, v2 170-220 k/mm2

Please refer to your kit-specific protocol for complete library denaturation and loading instructions.


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