What indications in the data suggest a failed library prep?

  1. Library preps that use 25 ng - 2 ug input RNA from a sample type that contains T-cells (e.g. PBLs, bone marrow, lymphoid organs), should generally produce greater than 4 nM final library, and up to hundreds of nM when using the Immunoverse TCR kits. Due to generally lower B-cell content in PBL, inputs of 100 ng - 3 μg RNA input from PBL should result in final library concentrations > 4 nM. If a library does not result in sufficient yield, the user should confirm the input contains RNA derived from T-cells or B-cells.
  2. After analyzing the sequencing results with Archer Analysis, there are several metrics that indicate a successful library prep and sequencing run. A user should expect that the % Reads Post Adapter Trimming is > 75% and that clonotypes are reported by the analysis pipeline.


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For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.