What are the criteria for fusion candidates to be displayed in the Strong Evidence tab?
If a fusion is in the Archer Quiver known fusion database, we always display it in the Strong Evidence tab.
In all other de-novo cases, the read event must meet the following criteria:
- Have a minimum of 5 unique (de-duplicated), breakpoint-spanning reads that support the gene fusion.
- Must not be flagged as a likely run cross-contamination event.
- At least 10% of the total reads for that GSP2 must support the fusion over the wild-type transcript.
- Have at least 3 unique start sites; breakpoint-spanning reads that support the gene fusion.
- Be an exon-exon fusion. Intron-exon fusions will be displayed in the weak evidence bin by default.
- Fusion partners cannot have homologous sequences. Fusion partners with too high sequence similarity will be flagged as likely mispriming events.
- Fusion partner genes must not be known paralogs.
- Fusion must pass additional alignment quality criteria (BLAST scores, mismatches in alignments, number of fusion partners involved, number of bases aligned to each fusion partner) to appear in the Strong Evidence bin.
These are the default values, however users can adjust these values in the Analysis User Settings based on their own data.
For Research Use Only. Not for use in diagnostic procedures.