What are the most common reasons runs fail QC?

Run QC is most affected by starting library quality, library complexity, and coverage. You will see the effects of these factors on “Average Unique Start Sites per GSP2” for VariantPlex assays, and "Average Unique Start Sites per GSP2 Control" for FusionPlex assays. This is found under the DNA/RNA Statistics section of the Read Statistics tab in Archer Analysis. The default setting is 50 Average Unique Start Sites per GSP2 for VariantPlex assays, and 10 Average Unique Start Sites per GSP2 Control for FusionPlex assays. These default values are adjustable, and you may want to change the cutoff based on your validation results.

DNA/RNA statistics in Archer Analysis


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For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.