No. ArcherDx’s Anchored Multiplex PCR (AMP) chemistry enables you to detect any fusion partner to your target of interest. You do, however, need to specify the exon of interest for each target as well as the direction of the fusion partner from the target exon (3’ or 5’). Note that mid-exon breakpoint primer design is not supported by Assay Designer at this time. If you want to detect mid-exon breakpoint fusions, contact technical support at email@example.com.
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