What extraction method should I use for my SureShot FFPE curls?

For all extraction methods below we recommend the following elution buffer and methods for quantification and QC:

Recommended Elution Buffer Quantification Method Recommended Quality Check Step
10mM Tris-HCl or nuclease-free water (No EDTA) Qubit RNA HS assay kit (Life Technologies® Q32852) Archer PreSeq RNA QC Assay (AK0043-16)

Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue

Recommended Extraction Method Extraction Kit Protocol Recommendations
Agencourt® FormaPure® Total Nucleic Acid Extraction (A33342)
  • DO NOT treat with DNase.
  • Use heat blocks versus water baths.
  • Perform a one-hour digestion at 55ºC at Step 5.
  • Perform reverse-crosslinking by incubating one hour at 80ºC between Steps 5 and 6.
  • Elute the sample in 40µL nuclease-free water at Step 23.
Qiagen® AllPrep DNA/RNA FFPE kit (80234)
  • Use QIAGEN Deparaffinization Solution (cat. no. 19093)
  • DO NOT treat with DNase.
  • Follow the RNA extraction protocol that DOES NOT include small RNAs.
  • Increase the crosslink reversal at 80°C from 15 minutes to 1 hour.
Covaris truXTRAC FFPE RNA Kit (520161)
  • Extend the reverse crosslinking step at 80°C to one hour.
Promega ReliaPrep™ FFPE Total RNA Miniprep System (Z1001)
  • Extend the reverse crosslinking step at 80°C to one hour.
Zymo Research Pinpoint™ Slide RNA Isolation System II (R1007)
  • Perform a one hour digestion at 55ºC followed by a one hour reverse crosslinking step at 80°C.


For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.

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For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.