We have found the PreSeq score to be around 23 when using the 2x BioRad reagents and the StepOne Plus qPCR instrument. The PreSeq score may vary depending on the type of mastermix and qPCR instrument used. Please see the links below for information on mastermixes and RNA PreSeq.
Archer PreSeq RNA QC Assay Tech Note under Publications and Posters.
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