Archer Analysis bioinformatics platform
NGS setup calculator


The easy way to calculate how much of your Archer library to load on a sequencer

This tool assists in the creation of a 40pM library pool ready for sequencing on an Illumina MiSeq® or Illumina NextSeq® instrument.

NextSeq/MiSeq loading pool

Desired loading volume (μL) 1000

1) Dilution of library pool to 4nM

Add input to dilute: 8.0 µL
Add H2O (µL): 2.0
Library Conc. (nM) NaOH Conc. (mM)
4 0.0


2) Denature 4nM library pool

Diluted input from Step 1: 10.0
200mM NaOH: 10.0

Incubate 5min at room temperature

Library Conc. (pM) NaOH Conc. (mM)
2000 100.0


3) Neutralize and dilute library pool to 40pM

Input with NaOH from Step 2 (µL): 20.0
200mM Tris, pH 7.0: 10 uL
HT1 Buffer: 970.0 uL
Library Conc. (pM) NaOH Conc. (mM)
40 2.0


4) Create aliquot to load onto MiSeq/NextSeq cartridge

Denatured pool from Step 3: 375.0 uL
PhiX 78.9 uL
HT1 Buffer: 546.1 uL
Final Pool (pM) Final NaOH (mM)
15 0.750


5) Load final pool into cartridge

This part is purely for your record-keeping

Print for your records

For Research Use Only. Not for use in diagnostic procedures.  For Research Use Only. Not for use in diagnostic procedures.

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Phone

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All content © 2018 ArcherDX, Inc.

For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.