Archer® SureShot™ ALK RET ROS1 controls are formalin-fixed, paraffin-embedded (FFPE) curls of homogeneously mixed, genetically defined ALK, RET and ROS1 fusion-positive cell lines developed in collaboration with Horizon Discovery, Ltd.. Curls contain EML4-ALK, CCDC6-RET, and SLC34A2-ROS1 fusions. When used alongside the Archer FusionPlex Panels containing ALK, RET, or ROS1 fusions, the SureShot controls are a powerful measure of extraction, library preparation, sequencing, and panel performance.
The Archer SureShot ALK, RET, ROS1 Positive Controls consist of 4 FFPE curls containing cell lines with physiologically relevant abundance of ALK, RET and ROS1 fusions, specifically:
Each curl contains a defined mixture of 3 different ALK, RET, and ROS1 fusion-positive cell lines along with a wild-type parental strain.
Libraries were created from material extracted from SureShot Positive Controls and the FusionPlex assays indicated in the graph below. The data reports the number of unique ALK, RET or ROS1 start sites detected using each FusionPlex assay across 7 replicates. The data supports the table information below, wherein any FusionPlex panel that includes ALK, RET and/or ROS1 primers detected the respective fusion in the SureShot Positive Control extractions. The dotted red line indicates the threshold criteria at which Archer Analysis software calls a "strong evidence", or valid fusion (3 unique start sites).
The table below indicates which FusionPlex panels include ALK, RET and/or ROS1 fusions, demonstrating the broad compatibility of the SureShot Positive Controls with FusionPlex panels.
|ALK, RET, ROS1 v2 panel||Learn more about the
FusionPlex ALK, RET, ROS1 v2 Panel
|Heme panel||Learn more about the
FusionPlex Heme Panel
|Sarcoma panel||Learn more about the
FusionPlex Sarcoma Panel
|Lung Thyroid panel||Learn more about the
FusionPlex Lung Thyroid Panel
|Solid Tumor panel||Learn more about the
FusionPlex Solid Tumor Panel
|Oncology Research panel||Learn more about the
FusionPlex Oncology Research Panel
|CTL panel||Learn more about the
FusionPlex CTL Panel
The Archer SureShot Negative Controls consist of 4 FFPE curls composed of 100% wild-type cells that carry no target gene fusion. These controls are suitable negative controls for all FusionPlex panels.
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