Archer SureShot™ Fusion Controls

FFPE Controls for ALK, RET, ROS1 Fusions

Archer® SureShot™ ALK RET ROS1 controls are formalin-fixed, paraffin-embedded (FFPE) curls of homogeneously mixed, genetically defined ALK, RET and ROS1 fusion-positive cell lines developed in collaboration with Horizon Discovery, Ltd.. Curls contain EML4-ALK, CCDC6-RET, and SLC34A2-ROS1 fusions. When used alongside the Archer FusionPlex Panels containing ALK, RET, or ROS1 fusions, the SureShot controls are a powerful measure of extraction, library preparation, sequencing, and panel performance.

Highlights

  • One FFPE sample, 3 fusion events
  • Suitable for use alongside multiple FusionPlex panels
  • Perfect for evaluations and lab optimizations
  • Negative controls are also available

Product Information

SK0109 - SureShot ARR Positive Fusion Controls

SK0110 - SureShot Negative Fusion Controls

SK0109 SureShot ARR Positive Fusion Controls SK0110 SureShot Negative Fusion Controls
FAQs

SDS


Positive Controls

Genetically defined fusion events

The Archer SureShot ALK, RET, ROS1 Positive Controls consist of 4 FFPE curls containing cell lines with physiologically relevant abundance of ALK, RET and ROS1 fusions, specifically:

  • EML4-ALK variant 1
  • ROS1-SL34A2
  • RET-CCDC6
GSP Primer Tubes

Each curl contains a defined mixture of 3 different ALK, RET, and ROS1 fusion-positive cell lines along with a wild-type parental strain.


Broad FusionPlex Panel Compatibility

Libraries were created from material extracted from SureShot Positive Controls and the FusionPlex assays indicated in the graph below. The data reports the number of unique ALK, RET or ROS1 start sites detected using each FusionPlex assay across 7 replicates. The data supports the table information below, wherein any FusionPlex panel that includes ALK, RET and/or ROS1 primers detected the respective fusion in the SureShot Positive Control extractions. The dotted red line indicates the threshold criteria at which Archer Analysis software calls a "strong evidence", or valid fusion (3 unique start sites).

SureShot Controls

The table below indicates which FusionPlex panels include ALK, RET and/or ROS1 fusions, demonstrating the broad compatibility of the SureShot Positive Controls with FusionPlex panels.


Negative Controls

The Archer SureShot Negative Controls consist of 4 FFPE curls composed of 100% wild-type cells that carry no target gene fusion. These controls are suitable negative controls for all FusionPlex panels.


Got Controls?

How to contact us

Address

2477 55th Street, Suite 202

Boulder, CO 80301

Phone

Phone: (877) 771 1093

Phone: (303) 357 9001

All content © 2017 ArcherDX, Inc.

For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.