Archer VariantPlex
Core Myeloid Kit

37 genes targeting critical AML, MPN and MDS markers

The VariantPlex Core Myeloid panel is a 37 gene targeted sequencing panel for critical AML, MPN and MDS markers. Powered by Anchored Multiplex PCR (AMP™) chemistry, the panel enables deep strand-specific amplification of molecular barcoded DNA fragments for Illumina® sequencing. After sequencing, Archer Analysis bioinformatics software deduplicates reads into unique starting molecules, correcting for sequencing and PCR-derived errors — enabling confident and sensitive mutation detection.

For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.

Highlights

  • Confident, molecular barcode driven variant detection
  • Robust FLT3-ITD detection of all sizes and insertion points
  • Outstanding coverage, including challenging genes like CEBPA
  • Simple, lyophilized single day library preparation

Product Information

AB0101 - VariantPlex® Core Myeloid Starter Kit, for Illumina® - 16 reactions

AB0100 - VariantPlex® Core Myeloid Kit, for Illumina® - 8 reactions

AB0102 - VariantPlex® Core Myeloid Kit, for Illumina® - 48 reactions


Illumina® Protocol Illumina® product insert
FAQs Archer Analysis
FLT3-ITD AMP tech note Get more info

VariantPlex Core Myeloid Kit features

37

Target genes

49kb

Total target size

>99%

Coverage uniformity > 20% mean

50-400ng

Input DNA required

3M

Recommended # of reads

Illumina®

Platform

FFPE, bone marrow, blood, PBL

Sample type

Input recommendations for FFPE samples varies depending on Archer Preseq™ DNA QC score. 50ng input recommended in absence of PreSeq screening



Archer VariantPlex Core Myeloid Kit targets

ABL1CSF3RGATA2NRASTET2
ANKRD26DDX41IDH1PHF6TP53
ASXL1DNMT3AIDH2PTPN11U2AF1
BCORETNK1JAK2RUNX1WT1
BRAFETV6KITSETBP1ZRSR2
CALREZH2KRASSF3B1
CBLFLT3MPLSRSF2
CEBPAGATA1NPM1STAG2

Don't see your gene?
Modify any panel with Archer Assay Designer.


VariantPlex Myeloid Kit capabilities

AMP-enabled FLT3-ITD detection

AMP-enabled FLT3-ITD detection

Independent and bidirectional probes are optimized to detect ITDs of all sizes.The reads originating from these probes are bioinformatically assembled by Archer Analysis to form longer consensus sequences that span the ITD. These longer sequences are then aligned to the reference genome to identify the presence of even very large ITDs.


High-complexity coverage across CEBPA

High-complexity coverage across CEBPA

Complete, strand-specific and bi-directional coverage of target exons, including traditionally difficult regions like CEBPA.


Robust CALR deletion detection

Robust CALR deletion detection

Powerful analysis of complex variants like CALR deletions can be detected using Archer Analysis due to the unique benefits of Anchored Multiplex PCR and extensive testing with positive samples and in-silico read generation technolog.





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Address

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Boulder, CO 80301

Phone

Phone: (877) 771 1093

Phone: (303) 357 9001

All content © 2017 ArcherDX, Inc.

For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.