Archer VariantPlex
Myeloid Kit

75 genes targeting critical AML, MPN, MDS and lymphoid markers

The VariantPlex Myeloid panel is a 75-gene targeted sequencing panel for AML, MPN, MDS and some Lymphoid malignancy markers. Powered by Anchored Multiplex PCR (AMP™) chemistry, the panel enables deep strand-specific amplification of molecular barcoded DNA fragments for Illumina Sequencing. After sequencing, Archer Analysis deduplicates reads into unique starting molecules, correcting for sequencing and PCR-derived errors--enabling sensitive and confident mutation detection.

For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.

Highlights

  • Confident and sensitive, unique molecule-driven variant detection
  • Robust FLT3-ITD detection of all sizes and insertion points
  • Outstanding coverage, including challenging genes like CEBPA
  • Simple, lyophilized single day library preparation
  • CNV detection for key genes in -7/7q, -5/5q, -13q, -11q, -12p/12p

Product Information

AB0109 - VariantPlex® Myeloid Starter Kit, for Illumina® - 16 reactions

AB0108 - VariantPlex® Myeloid Kit, for Illumina® - 8 reactions


Illumina® protocol
FAQs Archer Analysis
FLT3-ITD AMP tech note Get a quote

VariantPlex Myeloid Kit features

75

Gene targets

125kb

Total target size

>95%

Coverage uniformity > 20% mean

50-200ng

Input DNA required

4M

Recommended # of reads

Illumina®

Platform

FFPE, bone marrow, blood, PBL

Sample type

Coverage uniformity and recommended number of reads expected.

input recommendations for FFPE samples varies depending on Archer Preseq® DNA QC score. 50ng input recommended in absence of PreSeq screening



VariantPlex Myeloid Kit targets

ABL1CEBPAGNASMYD88SF3B1
ANKRD26CSF3RHRASNF1SH2B3
ASXL1CUX1IDH1NOTCH1SLC29A1
ATRXCXCR4IDH2NPM1SMC1A
BCORDCKIKZF1NRASSMC3
BCORL1DDX41JAK2PDGFRASRSF2
BRAFDHX15JAK3PHF6STAG2
BTKDNMT3AKDM6APPM1DSTAT3
CALRETNK1KITPTENTET2
CBLETV6KMT2APTPN11TP53
CBLBEZH2KRASRAD21U2AF1
CBLCFBXW7LUC7L2RBBP6U2AF2
CCND2FLT3MAP2K1RPS14WT1
CDC25CGATA1MPLRUNX1XPO1
CDKN2AGATA2MYCSETBP1ZRSR2

Genes in red are expected targets for CNV detection.

Don't see your gene?
Modify any panel with Archer Assay Designer.


VariantPlex Myeloid Kit capabilities

AMP-enabled FLT3-ITD detection

AMP-enabled FLT3-ITD detection

Independent and bidirectional probes are optimized to detect ITDs of all sizes.The reads originating from these probes are bioinformatically assembled by Archer Analysis to form longer consensus sequences that span the ITD. These longer sequences are then aligned to the reference genome to identify the presence of even very large ITDs.


High-complexity coverage across CEBPA

High-complexity coverage across CEBPA

Complete, strand-specific and bi-directional coverage of target exons, including traditionally difficult regions like CEBPA.


Robust CALR deletion detection

Robust CALR deletion detection

Powerful analysis of complex variants like CALR deletions can be detected using Archer Analysis due to the unique benefits of Anchored Multiplex PCR and extensive testing with positive samples and in-silico read generation technolog.


Sensitive and intuitive CNV detection

Sensitive and intuitive CNV detection

Chromosomal arm loss is prevalent in certain myeloid malignancies (e.g., -7/7q, -5/5q, -13q, -11q and -12p/12p). The VariantPlex Myeloid panel targets key genes within these chromosomes to indicate copy loss.





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How to contact us

Address

2477 55th Street, Suite 202

Boulder, CO 80301

Phone

Phone: (877) 771 1093

Phone: (303) 357 9001

All content © 2017 ArcherDX, Inc.

For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.