Archer VariantPlex
Myeloid Kit

75 genes targeting critical AML, MPN, MDS and lymphoid markers

The VariantPlex Myeloid panel is a 74-gene targeted sequencing panel for AML, MPN, MDS and some Lymphoid malignancy markers. Powered by Anchored Multiplex PCR (AMP™) chemistry, the panel enables deep strand-specific amplification of molecular barcoded DNA fragments for Illumina Sequencing. After sequencing, Archer Analysis deduplicates reads into unique starting molecules, correcting for sequencing and PCR-derived errors--enabling sensitive and confident mutation detection.

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For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.

Highlights

  • Confident and sensitive, unique molecule-driven variant detection
  • Robust FLT3-ITD detection of all sizes and insertion points
  • Outstanding coverage, including challenging genes like CEBPA
  • Simple, lyophilized single day library preparation
  • CNV detection for key genes in -7/7q, -5/5q, -13q, -11q, -12p/12p

Product Information

AB0109 - Variantplex® Myeloid Starter Kit, For Illumina® - 16 reactions

AB0108 - Variantplex® Myeloid Kit, For Illumina® - 8 reactions


Illumina® protocol
FAQs Archer Analysis
FLT3-ITD AMP tech note Get more info

Watch the video: FLT3-ITD detection with VariantPlex Myeloid

The molecular landscape of leukemia and lymphoma has expanded exponentially in the last two decades, and the complexity and scope of biomarkers makes molecular analysis difficult for any single approach. The Archer VariantPlex Myeloid assay is powered by Anchored Multiplex PCR (AMP™) target enrichment chemistry to detect multiple mutation types and gene expression profiling in a single sample.

 




VariantPlex Myeloid Kit Features

75

Gene targets

125kb

Total target size

>95%

Coverage uniformity > 20% mean

50-200ng

Input DNA required

4M

Recommended # of reads

Illumina®

Platform

FFPE, bone marrow, blood, PBL

Sample type

Coverage uniformity and recommended number of reads expected.

input recommendations for FFPE samples varies depending on Archer Preseq™ DNA QC score. 50ng input recommended in absence of PreSeq screening



Archer VariantPlex Myeloid Kit Targets

ABL1CEBPAGNASMYD88SF3B1
ANKRD26CSF3RHRASNF1SH2B3
ASXL1CUX1IDH1NOTCH1SLC29A1
ATRXCXCR4IDH2NPM1SMC1A
BCORDCKIKZF1NRASSMC3
BCORL1DDX41JAK2PDGFRASRSF2
BRAFDHX15JAK3PHF6STAG2
BTKDNMT3AKDM6APPM1DSTAT3
CALRETNK1KITPTENTET2
CBLETV6KMT2APTPN11TP53
CBLBEZH2KRASRAD21U2AF1
CBLCFBXW7LUC7L2RBBP6U2AF2
CCND2FLT3MAP2K1RPS14WT1
CDC25CGATA1MPLRUNX1XPO1
CDKN2AGATA2MYCSETBP1ZRSR2

Genes in red are expected targets for CNV detection.

* CNV detection only.

Need to modify a panel?

AMP chemistry is flexible and gene-level probe sets are modular. This means that any of our 94 wet lab-validated designs can be added to any preconfigured panel to build assays that fit your research goals. Archer Assay Designer gives you the power to easily determine assay content and keep current with the latest research.



Want to get more details on the VariantPlex Myeloid Kit?

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If you have any technical questions, please reach out to our expert Technical Support staff through tech@archerdx.com or by calling ArcherDX directly at +1.303.357.9001.

How to contact us

Address

2477 55th Street, Suite 202

Boulder, CO 80301

Phone

Phone: (877) 771 1093

Phone: (303) 357 9001

All content © 2017 ArcherDX, Inc.

For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.