Archer VariantPlex
Myeloid Kit

74 genes targeting critical AML, MPN, MDS and lymphoid markers

The VariantPlex Myeloid panel is a 74-gene targeted sequencing panel for AML, MPN, MDS and some Lymphoid malignancy markers. Powered by Anchored Multiplex PCR (AMP™) chemistry, the panel enables deep strand-specific amplification of molecular barcoded DNA fragments for Illumina Sequencing. After sequencing, Archer Analysis deduplicates reads into unique starting molecules, correcting for sequencing and PCR-derived errors--enabling sensitive and confident mutation detection.
For Research Use Only. Not for use in diagnostic procedures.

Highlights

  • Confident and sensitive, unique molecule-driven variant detection
  • Robust FLT3-ITD detection of all sizes and insertion points
  • Outstanding coverage, including challenging genes like CEBPA
  • Simple, lyophilized single day library preparation
  • CNV detection for key genes in -7/7q, -5/5q, -13q, -11q, -12p/12p

Product Information

FAQs Archer Analysis

Watch the video: FLT3-ITD Detection With Archer® Blood Cancer Assays

The molecular landscape of leukemia and lymphoma has expanded exponentially in the last two decades, and the complexity and scope of biomarkers makes molecular analysis difficult for any single approach. Archer Blood Cancer assays are powered by Anchored Multiplex PCR (AMP™) target enrichment chemistry to detect multiple mutation types and gene expression profiling in a single sample.

 




VariantPlex Myeloid Kit Features

74

Gene targets

125kb

Total target size

>95%

Coverage uniformity > 20% mean†

2.5 hours

Hands-on time

50-200ng‡

Input DNA required

4M

Recommended # of reads†

6 hours

Time required

99%

On-target

Illumina®

Platform

FFPE, bone marrow, blood, PBL

Sample type

†Coverage uniformity and recommended number of reads expected.

‡input recommendations for FFPE samples varies depending on Archer Preseq™ DNA QC score. 50ng input recommended in absence of PreSeq screening



Archer VariantPlex Myeloid Kit Targets

ABL1CEBPAGNASMYD88SH2B3
ANKRD26CSF3RHRASNF1SLC29A1
ASXL1CUX1IDH1NOTCH1SMC1A
ATRXCXCR4IDH2NPM1SMC3
BCORDCKIKZF1NRASSRSF2
BCORL1DDX41JAK2PDGFRASTAG2
BRAFDHX15JAK3PHF6STAT3
BTKDNMT3AKDM6APPM1DTET2
CALRETNK1KITPTENTP53
CBLETV6KMT2APTPN11U2AF1
CBLBEZH2KRASRAD21U2AF2
CBLCFBXW7LUC7L2RBBP6WT1
CCND2FLT3MAP2K1RPS14XPO1
CDC25CGATA1MPLRUNX1ZRSR2
CDKN2AGATA2MYCSF3B1

Genes in red are expected targets for CNV detection

Need to modify a panel?

AMP chemistry is flexible and gene-level probe sets are modular. This means that any of our 94 wet lab-validated designs can be added to any preconfigured panel to build assays that fit your research goals. Archer Assay Designer gives you the power to easily determine assay content and keep current with the latest research.



Want to get more details on the VariantPlex Myeloid Kit?

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If you have any technical questions, please reach out to our expert Technical Support staff through tech@archerdx.com or by calling ArcherDX directly at +1.303.357.9001.

How to contact us

Address

2477 55th Street, Suite 202

Boulder, CO 80301

Phone

Phone: (877) 771 1093

Phone: (303) 357 9001

All content © 2017 ArcherDX, Inc.

For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.