Anchored Multiplex PCR Enables Sensitive NGS-Based Mutation Detection in the Context of Large Primer Panels

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Association for Molecular Pathology (AMP) | San Antonio, USA 2018

Verity Johnson, Kaitlyn E. Moore, Laura Griffin, Abel Licon, Aaron Berlin, Ryan Walters. ArcherDX, Boulder, CO. USA.

The mutational landscape of pediatric and adult cancers is changing at a rapid pace. Unfortunately, some targeted high-throughput sequencing assays lack the flexibility required to quickly incorporate new discoveries. Adding new targets to existing panels often requires re-optimization, and may risk reductions in variant detection sensitivity. Here, we describe a target enrichment method, Anchored Multiplex PCR (AMP™) that performs consistently regardless of panel size and content, which enables modularity in panel design and confidence in assay adaptability as the field advances.

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