Assay Designer

Design your own panel with genes of interest for targeted next-generation sequencing (NGS) using RNA, DNA or ctDNA. Archer® Assay Designer provides the flexibility to design an assay from scratch using your own list of genes or modify an existing Archer catalog or Focus panel.

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Key Features
Expert Primer Design

Primer design is the foundation of next-generation sequencing (NGS)-based assay performance, and our in-house design experts leverage the design flexibility of Anchored Multiplex PCR (AMP™) chemistry to ensure that all primers generated in Assay Designer deliver high uniformity and low off-targeting.

Simple and INtuitive

Assay Designer has a simple, intuitive interface to help you quickly build the target panel that you want. Browse through hundreds of wet lab-tested and verified designs, or use state-of-the-art primer design tools to build the assay to fit your needs.

Exceptional Performance

Assay Designer boasts cutting-edge primer design software and an expansive internal knowledge base of target designs and validated primer sets. Designed with maximum flexibility and unparalleled performance in mind, you can be assured that your targets will achieve complete coverage.

Need a place to start?
Save some time with an Archer Focus panel.

What’s your focus?

All Archer panels are easily modified, however, Archer Focus panels make great building-blocks for your custom assay. They are small, disease-specific panels of pre-validated targets that are made to order. Each can be used as a starting point in Assay Designer to accommodate your needs.

Focus panels are great starting points for building customized panels.

VariantPlex® TP53 Assay

1 Gene

TP53

Illumina®

100K Recommended Read Depth

Highlights

  • Maximum TP53 coverage
  • Optimized for full exon coverage with a low number of sequencing reads

VariantPlex
BRCA v2

2 Genes

BRCA1, BRCA2

Illumina

500K Recommended Read Depth

Highlights

  • Deep coverage over all coding regions
  • Confident de novo variant detection

VariantPlex
BRCA and PALB2 Focus

3 Genes

BRCA1, BRCA2, PALB2

Illumina

500K Recommended Read Depth

Highlights

  • Rapid, lyophilized workflow
  • Complete coding region coverage

FusionPlex® Lung Focus

5 Genes

ALK, RET, ROS1, NTRK1, MET

Illumina and Ion Torrent™

500K Recommended Read Depth

Highlights

  • Highly focused detection of oncogenic fusions in lung cancer
  • Includes select insertions and point mutations in ALK and RET, including known resistant point mutations
  • Captures MET exon 14 skipping events

FusionPlex
Brain Focus

10 Genes

BRAF, EGFR, EWSR1, FGFR3, MYB, NTRK1, NTRK2, NTRK3, PDGFB, PDGFRA

Illumina and Ion Torrent

500K Recommended Read Depth

Highlights

  • RNA fusion detection for common brain tumor translocations
  • Allows for initial sample stratification across pediatric and adult brain tumor samples resulting from fusion events
  • Captures prominent BRAF, EGFR and PDGFRA mutations

FusionPlex
Myeloid Focus

9 Genes

RUNX1, RARA, NUP214, NUP98, CBFB, ABL1, BCR, RBM15, KMT2A

Illumina and Ion Torrent

500K Recommended Read Depth

Highlights

  • Coverage of critical myeloid fusion targets, including ABL1 resistant point mutations
  • High degree of multiplexing (1M reads)
  • Novel fusion detection

VariantPlex AML Focus

11 Genes

ASXL1, CEBPA, DNMT3A, FLT3, IDH1, IDH2, JAK2, panel, NPM1, RUNX1, TP53

Illumina

750K Recommended Read Depth

Highlights

  • Robust FLT3-ITD detection
  • Deep, full coverage of CEBPA

LiquidPlex™
Lung Focus

12 Genes

Hotspots in:
ALK, AKT1, BRAF, CTNNB1, EGFR, ERRB2, KRAS, MAP2K1, MET, NRAS, PIK3CA, ROS1

Illumina

1M Recommended Read Depth

Highlights

  • Simple, lyophilized workflow
  • MBC-enabled error correction and low-allele fraction (AF) detection

VariantPlex
Solid Tumor Focus

20 Genes

AKT1, BRAF, EGFR, ERBB2, FOXL2, GNA11, GNAQ, GNAS, HRAS, IDH1, IDH2, panel, KRAS, MET, NRAS, PDGFRA, PIK3CA, RET, TERTp, TP53

Illumina

750K Recommended Read Depth

Highlights

  • Built for challenging FFPE samples
  • MBC-powered variant detection

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