Clinical Tumor Sequencing:
Applications and considerations for targeted sequencing using Anchored Multiplex PCR

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Patrick Hurban, Ph.D., Senior Director and Global Head of Genomic Development & Esoteric Assays at Q2 Solutions.

Webinar abstract

Targeted sequencing of cancer genomes is a powerful tool for clinical development of targeted therapeutics, enabling comprehensive mutation profiling from low-input FFPE-preserved tumor samples. Anchored Multiplex PCR (AMP™) is a target enrichment chemistry for next-generation sequencing (NGS) that is well suited for sequencing of FFPE specimens, as the molecular barcoded adaptors utilized in AMP preserve library complexity of low-input, degraded samples. Unidirectional gene-specific primers permit open-ended capture of nucleic acid fragments for NGS-based identification of known and novel mutations, including SNVs/indels, copy number variants and gene fusions.

In this webinar, Dr. Hurban will review multiple applications of AMP-based NGS performed in a global CRO, discussing assay suitability and performance for multiple disease and mutation types.

This webinar is co-sponsored by

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About Patrick Hurban, Ph.D.

Patrick Hurban

Dr. Hurban is the Senior Director and Global Head of Genomic Development & Esoteric Assays at Q2 Solutions. In this role, he is responsible for the identification and implementation of new genomic capabilities as well as the development and validation of genomic assays to support research and clinical programs. These include broad-based screening assays, such as exome and RNA sequencing, to highly focused expression, genotyping and sequencing assays that target a smaller number of genes.

Dr. Hurban has more than 25 years of experience in molecular genetics, including more than 15 years in positions of increasing responsibility in high-profile genomics-focused organizations. His research interests have focused on the genetic control of gene expression and have spanned diverse fields such as toxicology, developmental, and cancer biology.

Following a Bachelor’s degree in Biology from the University of North Carolina at Chapel Hill, he earned his Ph.D. in Human Genetics from the University of Utah in Salt Lake City before serving as an American Cancer Society postdoctoral research fellow in the Department of Developmental Biology at Stanford University.

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For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.