Heme v2


The Archer® FusionPlex Heme v2 panel is an ribonucleic acid (RNA)-based targeted sequencing test that detects and identifies fusions of 87 genes associated with hematological malignancies. Using Archer’s proprietary Anchored Multiplex PCR (AMP™)-based enrichment, fusions of all genes in this panel can be identified in a single sequencing test, even without prior knowledge of fusion partners or breakpoints. Additionally, select genes are targeted for molecular barcode-enabled relative expression level detection and point mutation coverage.
Key Features
Comprehensive fusion detection

AMP chemistry utilizes open-ended targeted amplification to identify gene fusions whether or not the fusion partner is known.

Simple, lyophilized workflow

Easy-to-handle lyophilized reagents and a simple workflow to minimize errors and reduce turn-around time.

Sample versatility

Wide sample type compatibility, including FFPE or fresh frozen tissue, blood, bone marrow aspirates, and cell fractions.

Expression profiling

Molecular barcoded fragments enable accurate RNA abundance calculations.

Product Information
Specifications and Performance

87

Gene Targets

> 10ng

Input Nucleic Acid Required*

> 90%

Unique Molecular On-Target %

2.5 hours

Hands-on Time

9 hours

Total Time

Illumina and
Ion Torrent

Platform

Blood, Fresh Frozen, FFPE

Sample Types

*Input recommendations for FFPE samples vary depending on Archer PreSeq® RNA QC score; 50ng input recommended in absence of PreSeq screening

Gene Targets

ABL1

ABL2

ALK

BCL11B

BCL2

BCL3

BCL6

BCR

BIRC3

CBFB

CCND1

CCND2

CCND3

CD274

CDK6

CDKN2A

CEBPA

CEBPD

CEBPE

CEBPG

CHD1

CHIC2

CIITA

CREBBP

CRLF2

CSF1R

CTLA4

DEK

DUSP22

EBF1

EIF4A1

EPOR

ERG

ETV6

FGFR1

FOXP1

GLIS2

ID4

IKZF1

IKZF2

IKZF3

IRF4

IRF8

JAK2

KAT6A

KLF2

KMT2A

MALT1

MECOM

MKL1

MLF1

MLLT10

MLLT4

MUC1

MYC

MYH11

NF1

NFKB2

NOTCH1

NTRK3

NUP214

NUP98

P2RY8

PAG1

PAX5

PDCD1

PDCD1LG2

PDGFRA

PDGFRB

PICALM

PML

PRDM16

PTK2B

RARA

RBM15

ROS1

RUNX1

RUNX1T1

SEMA6A

SETD2

STIL

TAL1

TCF3

TFG

TP63

TYK2

ZCCHC7

LEGEND

SNV/Indel
Fusion, splicing or exon-skipping
Expression

Note: Fusions involving BCR and TCR loci, including IGH, IGL and IGK, are targeted for expression and may not be explicitly called as a fusion because these often do not result in chimeric transcripts.

NeeD to Modify Your Panel?

Add any of our wet lab-validated designs to this panel with Archer Assay Designer to build an assay that fits your exact requirements.

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