NGS Panels for Liquid Biopsy Samples


The Archer® Reveal ctDNA™ 28 panel for Illumina® is an advanced and user-friendly solution for targeted next-generation sequencing (NGS) of circulating cell-free tumor DNA (ccfDNA/cfDNA/ctDNA) from 28 genes commonly found mutated in solid tumor type cancers.
Key Features
Relevant fragment capture

Anchored Multiplex PCR (AMP™)-based target enrichment preferentially enriches for highly fragmented ctDNA fragments over genomic cell-free DNA (cfDNA) to reduce background and increase detection sensitivity of low-allelic fraction (AF) mutations

Single-day library prep

NGS-ready libraries can be prepared in under 8 hours with less than 1 hour of hands-on time, providing sequencing results in as little as 3 days.

Error correction & quantitative analysis

Unique molecular barcode sequences ligated prior to amplification enable duplicate read binning and advanced error correction for confident variant calls.

Sensitive & robust variant detection

Reliably detect high-AF (5%) variants using as little as 5ng total cfDNA input and low AF (1%) variants from as little as 10ng.

Specifications and Performance

28

Gene Targets

≥5ng

Input nucleic acid required*

> 10ng

Input Nucleic Acid Required*

1.5 million

Recommended # of Reads

100%

Target Bases Covered

>90%

Raw Molecular On-Target

1 hour

Hands-On Time

1 day

Total Time

Illumina®

Platform

Gene Targets

LEGEND

Single nucleotide variant (SNV)/Indel SNV/Indel
Expression Expression
Fusion Fusion, splicing or exon-skipping
Internal tandem duplication (ITD) Internal tandem duplication (ITD)
Copy number variation (CNV) CNV

NeeD to Modify Your Panel?

Add any of our wet lab-validated designs to this panel with Archer Assay Designer to build an assay that fits your exact requirements.

  • Find Your Fit

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