Pan-Heme


The Archer® FusionPlex Pan-Heme panel is an Anchored Multiplex PCR (AMP™)-based next-generation sequencing (NGS) test to detect and identify fusions, point mutations and expression levels from ribonucleic acid (RNA) input. The panel encompasses all targets in the FusionPlex heme panels, amounting to over 199 genes relating to lymphoid and myeloid malignancies. By using gene-specific primers to amplify into molecular barcodes ligated onto the cDNA fragment ends, both known and novel fusions can be identified.
Key Features
Comprehensive fusion detection

Including known and novel fusion events.

Efficient, lyophilized workflow

Targets key hotspots regions for simultaneous point mutation detection.

Expression profiling

Molecular barcode-enabled expression detection for key genes including immune checkpoint and DLBCL subtyping.

Confirm fusion events

Expression imbalance enabled in select genes for secondary fusion confirmation.

Specifications and Performance

199

Gene Targets

> 10ng

Input Nucleic Acid Required*

> 90%

Unique Molecular On-Target %

2.5 hours

Hands-on Time

9 hours

Total Time

Illumina and
Ion Torrent

Platform

Blood, Fresh Frozen, FFPE

Sample Types

*Input recommendations for FFPE samples vary depending on Archer PreSeq® RNA QC score; 50ng input recommended in absence of PreSeq screening

Gene Targets

LEGEND

SNV/Indel
Expression
Fusion, splicing or exon-skipping

Notes

  • Fusions involving BCR and TCR loci, including IGH, IGL and IGK, are targeted for expression and may not be explicitly called as a fusion because these often do not result in chimeric transcripts
  • Targets are listed for primary purpose but may detect other mutation types
  • ABC and GCB DLBCL signature genes are targeted for expression. An experimental classification tool for ABC/GCB differentiation is available in Archer Analysis
 
NeeD to Modify Your Panel?

Add any of our wet lab-validated designs to this panel with Archer Assay Designer to build an assay that fits your exact requirements.

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