Heme V2

The FusionPlex Heme v2 panel is an ribonucleic acid (RNA)-based targeted next-generation sequencing (NGS) product that detects and identifies fusions of 87 genes associated with hematological malignancies. Using proprietary Anchored Multiplex PCR (AMP™) -based enrichment, fusions of all genes in this panel can be identified in a single sequencing assay, even without prior knowledge of fusion partners or breakpoints. Additionally, select genes are targeted for molecular barcode-enabled relative expression level detection and point mutation coverage.

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Comprehensive Fusion Detection

AMP chemistry utilizes open-ended targeted amplification to identify gene fusions whether or not the fusion partner is known.

Splice Variants

Wide sample type compatibility, including FFPE or fresh frozen tissue, blood, bone marrow aspirates and cell fractions.

Expression Profiling

Molecular barcoded fragments enable accurate RNA abundance calculations.

Simple, Lyophilized Workflow

Easy-to-handle lyophilized reagents and a simple workflow to minimize errors and reduce turn-around time.

Properties

Specifications

Gene Targets

87 Input DNA Required

≤10ng

Raw Molecular On-Target

> 90%

Hands-On Time

2.5 Hour

Total Time

9 Hours

Platform

Illumina® and Ion Torrent®

Sample Types

Blood, Fresh frozen and FFPE

*Input recommendations for FFPE samples vary depending on PreSeq® RNA QC score; 50ng input recommended in absence of PreSeq screening

LEGEND

SNV/Indel
Fusion, splicing or exon-skipping
Expression

Note: Fusions involving BCR and TCR loci, including IGH, IGL and IGK, are targeted for expression and may not be explicitly called as a fusion because these often do not result in chimeric transcripts.

Product Information

Illumina®

Ion Torrent™

Archer® Analysis

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Customize this panel by adding any of our functionally-tested designs. Assay Marketplace lets you browse our catalog of targeted AMP chemistry-based panels, modify an existing panel with additional targets, or create a new panel that fits your exact requirements.

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For Research Use Only. Not for use in diagnostic procedures.

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