Lymphoma

The FusionPlex Lymphoma panel is a targeted next-generation sequencing (NGS) product to simultaneously detect and identify fusions, point mutations, and expression levels in 125 genes linked to lymphomas. Using Anchored Multiplex PCR (AMP™)-based enrichment, fusions can be identified even without prior knowledge of fusion partners or breakpoints. Additionally, select genes are targeted for molecular barcode-enabled relative expression detection and coverage over key hotspot regions.

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Comprehensive Fusion Detection

AMP chemistry utilizes open-ended targeted amplification to identify gene fusions whether or not the fusion partner is known.

Low Input

Purpose-built for wide sample type compatibility, including low-input and degraded samples such as FFPE tissue.

Expression Profiling

Molecular barcoded fragments enable accurate RNA abundance calculations.

Simple, Lyophilized Workflow

Easy-to-handle lyophilized reagents and a simple workflow to minimize errors and reduce turn-around time.

Properties

Specifications

Gene Targets

125 Input Nucleic Acid Required*

≤10ng

Unique Molecular On-Target %

>90%

Hands-on Time

2.5 hours

Total Time

9 hours

Platform

Illumina® and Ion Torrent®

Sample Types

Blood, Fresh frozen and FFPE

*Input recommendations for FFPE samples vary depending on PreSeq® RNA QC score; 50ng input recommended in absence of PreSeq screening

LEGEND

SNV/Indel
Fusion, splicing or exon-skipping
Expression

Notes:

Fusions involving BCR and TCR loci, including IGH, IGL and IGK, are targeted for expression and may not be explicitly called as a fusion because these often do not result in chimeric transcripts
Targets are listed for primary purpose but may detect other mutation types
ABC and GCB DLBCL signature genes are targeted for expression. An experimental classification tool for ABC/GCB differentiation is available in Archer Analysis

Product Information

Illumina®

Ion Torrent™

Archer® Analysis

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Customize this panel by adding any of our functionally-tested designs. Assay Marketplace lets you browse our catalog of targeted AMP chemistry-based panels, modify an existing panel with additional targets, or create a new panel that fits your exact requirements.

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For Research Use Only. Not for use in diagnostic procedures.

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