Lymphoma

The Archer® FusionPlex Lymphoma panel is a targeted next-generation sequencing (NGS) product to simultaneously detect and identify fusions, point mutations, and expression levels in 125 genes linked to lymphomas. Using Anchored Multiplex PCR (AMP™)-based enrichment, fusions can be identified even without prior knowledge of fusion partners or breakpoints. Additionally, select genes are targeted for molecular barcode-enabled relative expression detection and coverage over key hotspot regions.

  • Key Features
  • Specifications
  • Gene Targets
  • Additional Resources
Comprehensive Fusion Detection

AMP chemistry utilizes open-ended targeted amplification to identify gene fusions whether or not the fusion partner is known.

Low Input

Purpose-built for wide sample type compatibility, including low-input and degraded samples such as FFPE tissue.

Expression Profiling

Molecular barcoded fragments enable accurate RNA abundance calculations.

Simple, Lyophilized Workflow

Easy-to-handle lyophilized reagents and a simple workflow to minimize errors and reduce turn-around time.

Properties

Specifications

Gene Targets
125
Input Nucleic Acid Required*
≤10ng
Unique Molecular On-Target %
>90%
Hands-on Time
2.5 hours
Total Time
9 hours
Platform
Illumina® and Ion Torrent®
Sample Types
Blood, Fresh frozen and FFPE

*Input recommendations for FFPE samples vary depending on Archer PreSeq® RNA QC score; 50ng input recommended in absence of PreSeq screening

AICDA

AKT3

ALK

ASB13

BATF3

BAX

BCL2

BCL2A1

BCL3

BCL6

BCR

BIRC3

BLNK

BMF

BMP7

BRAF

BTK

CARD11

CBFB

CCDC50

CCND1

CCND2

CCND3

CD274

CD44

CD79B

CDC25A

CDK6

CDKN2A

CDKN2B

CEBPD

CEBPE

CEBPG

CHIC2

CIITA

CREB3L2

CREBBP

CTLA4

CYB5R2

DEK

DENND3

DLEU1

DNMT3A

DNMT3B

DNTT

DUSP22

E2F2

EIF4A1

ENTPD1

ETV6

EXOC2

EZH2

FAM216A

FBXW7

FGFR1

FOXP1

FUT8

IDH1

IDH2

IL16

IRF4

IRF8

ITPKB

JAK1

JAK2

JAK3

KIAA0101

KMT2A

KRAS

LIMD1

LMO2

LRMP

LZTS1

MAL

MALT1

MAML3

MKL1

MLF1

MLLT10

MME

MUC1

MYBL1

MYC

MYD88

NEK6

NFKB1

NFKB2

NME1

NOTCH1

NOTCH2

NRAS

P2RY8

PAICS

PDCD1

PDCD1LG2

PDGFRA

PIM1

PIM2

PLCG1

PLCG2

PPAT

PRDM16

PRKAR2B

PTPN1

PYCR1

RAB29

RAG1

RAG2

RANBP1

RHOA

S1PR2

SERPINA9

SF3B1

SH3BP5

STAT3

STAT5B

STAT6

STIL

STRBP

TCF3

TNFRSF13B

TNFSF4

TP63

WT1

XPO1

LEGEND

SNV/Indel
Fusion, splicing or exon-skipping
Expression

Notes:

  • Fusions involving BCR and TCR loci, including IGH, IGL and IGK, are targeted for expression and may not be explicitly called as a fusion because these often do not result in chimeric transcripts
  • Targets are listed for primary purpose but may detect other mutation types
  • ABC and GCB DLBCL signature genes are targeted for expression. An experimental classification tool for ABC/GCB differentiation is available in Archer Analysis
Product Information
Illumina
  • AB0019 - 8-reactions
  • AB0071 - 48-reactions
  • AB0041 – 16-reactions – Ask about our starter panel
ION TORRENT™
  • AB0020 - 8-reactions
  • AB0072 - 48-reactions
  • AB0042 – 16-reactions – Ask about our starter panel
Archer® Analysis

Need to modify your panel?

Add any of our wet lab-validated designs to this panel with Archer Assay Designer to build an assay that fits your exact requirements.

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For Research Use Only. Not for use in diagnostic procedures.

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For Research Use Only. Not for use in diagnostic procedures.