FusionPlex Pan-Heme

Pan-Heme

The FusionPlex Pan-Heme panel is an Anchored Multiplex PCR (AMP™)-based next-generation sequencing (NGS) product to detect and identify fusions, point mutations and expression levels from ribonucleic acid (RNA) input. The panel encompasses all targets in the FusionPlex heme panels, amounting to over 199 genes relating to lymphoid and myeloid malignancies. By using gene-specific primers to amplify into molecular barcodes ligated onto the cDNA fragment ends, both known and novel fusions can be identified.

  • Key Features
  • Specifications
  • Gene Targets
  • Additional Resources
Comprehensive Fusion Detection

AMP chemistry utilizes open-ended targeted amplifications to identify gene fusions whether or not the fusion partner is know.

Sample Versatility

Wide sample type compatibility, including FFPE or fresh frozen tissue, blood, bone marrow aspirates, and cell fractions.

Expression Profiling

Molecular barcoded fragments enable accurate RNA abundance calculations.

Simple, Lyophilized Workflow

Easy-to-handle lyophilized reagents and a simple workflow to minimize errors and reduce turn-around time.

Properties

Specifications

Gene Targets
199
Input Nucleic Acid Required
≤10ng
Unique Molecular On-Target%
90%
Hands-on Time
2.5 hours
Total Hours
9 hours
Platform
Illumina® and Ion Torrent
Sample Types
Blood, Fresh Frozen, FFPE

Input recommendations for FFPE samples vary depending on PreSeq® RNA QC score; 50ng input recommended in absence of PreSeq screening

Product Information
Illumina
ION TORRENT™
Archer® Analysis

ABL1

ABL2

AICDA

AKT3

ALK

ASB13

ASXL1

BATF3

BAX

BCL11B

BCL2

BCL2A1

BCL3

BCL6

BCR

BIRC3

BLNK

BMF

BMP7

BRAF

BTK

CALR

CARD11

CBFB

CBL

CCDC50

CCND1

CCND2

CCND3

CD274

CD44

CD79B

CDC25A

CDK6

CDKN2A

CDKN2B

CEBPA

CEBPD

CEBPE

CEBPG

CHD1

CHIC2

CIITA

CREB3L2

CREBBP

CRLF2

CSF1R

CSF3R

CTLA4

CYB5R2

DCK

DEK

DENND3

DLEU1

DNM2

DNMT3A

DNMT3B

DNTT

DUSP22

E2F2

EBF1

EIF4A1

ENTPD1

EPOR

ERG

ETV6

EXOC2

EZH2

FAM216A

FBXW7

FGFR1

FGFR2

FGFR3

FLT3

FOXP1

FUT8

GATA1

GATA2

GLIS2

GNAS

HOXA10

HOXA9

ID4

IDH1

IDH2

IKZF1

IKZF2

IKZF3

IL16

IL7R

IRF4

IRF8

ITPKB

JAK1

JAK2

JAK3

KAT6A

KDM6A

KIAA0101

KIT

KLF2

KMT2A

KRAS

LIMD1

LMO1

LMO2

LRMP

LYL1

LZTS1

MAL

MALT1

MAML3

MECOM

MKL1

MLF1

MLLT10

MLLT4

MME

MPL

MUC1

MYBL1

MYC

MYD88

MYH11

NEK6

NF1

NFKB1

NFKB2

NME1

NOTCH1

NOTCH2

NPM1

NRAS

NT5C2

NTRK3

NUP214

NUP98

P2RY8

PAG1

PAICS

PAX5

PBX1

PDCD1

PDCD1LG2

PDGFRA

PDGFRB

PHF6

PICALM

PIM1

PIM2

PLCG1

PLCG2

PML

PPAT

PRDM16

PRKAR2B

PTK2B

PTPN1

PTPN11

PYCR1

RAB29

RAG1

RAG2

RANBP1

RARA

RBM15

RHOA

ROS1

RUNX1

RUNX1T1

S1PR2

SEMA6A

SERPINA9

SETBP1

SETD2

SF3B1

SH2B3

SH3BP5

SLC29A1

SOX11

SRSF2

STAT3

STAT5B

STAT6

STIL

STRBP

TAL1

TCF3

TFG

TLX1

TLX3

TNFRSF13B

TNFSF4

TP63

TYK2

U2AF1

WT1

XPO1

ZCCHC7

LEGEND

SNV/Indel
Fusion, splicing or exon-skipping
Expression

Notes:

  • Fusions involving BCR and TCR loci, including IGH, IGL and IGK, are targeted for expression and may not be explicitly called as a fusion because these often do not result in chimeric transcripts
  • Targets are listed for primary purpose but may detect other mutation types
  • ABC and GCB DLBCL signature genes are targeted for expression. An experimental classification tool for ABC/GCB differentiation is available in Archer Analysis
 

Need to modify your panel?

Add any of our wet lab-validated designs to this panel with Archer Assay Designer to build an assay that fits your exact requirements.

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For Research Use Only. Not for use in diagnostic procedures.

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For Research Use Only. Not for use in diagnostic procedures.