Pan-Heme

The FusionPlex Pan-Heme panel is an Anchored Multiplex PCR (AMP™)-based next-generation sequencing (NGS) product to detect and identify fusions, point mutations and expression levels from ribonucleic acid (RNA) input. The panel encompasses all targets in the FusionPlex heme panels, amounting to over 199 genes relating to lymphoid and myeloid malignancies. By using gene-specific primers to amplify into molecular barcodes ligated onto the cDNA fragment ends, both known and novel fusions can be identified.

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Comprehensive Fusion Detection

AMP chemistry utilizes open-ended targeted amplifications to identify gene fusions whether or not the fusion partner is know.

Sample Versatility

Wide sample type compatibility, including FFPE or fresh frozen tissue, blood, bone marrow aspirates, and cell fractions.

Expression Profiling

Molecular barcoded fragments enable accurate RNA abundance calculations.

Simple, Lyophilized Workflow

Easy-to-handle lyophilized reagents and a simple workflow to minimize errors and reduce turn-around time.

Properties

Specifications

Gene Targets

199 Input Nucleic Acid Required

≤10ng

Unique Molecular On-Target%

90%

Hands-on Time

2.5 hours

Total Hours

9 hours

Platform

Illumina® and Ion Torrent

Sample Types

Blood, Fresh Frozen, FFPE

Input recommendations for FFPE samples vary depending on PreSeq® RNA QC score; 50ng input recommended in absence of PreSeq screening

Product Information

Illumina®

Ion Torrent™

Archer® Analysis

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LEGEND

SNV/Indel
Fusion, splicing or exon-skipping
Expression

Notes:

Fusions involving BCR and TCR loci, including IGH, IGL and IGK, are targeted for expression and may not be explicitly called as a fusion because these often do not result in chimeric transcripts
Targets are listed for primary purpose but may detect other mutation types
ABC and GCB DLBCL signature genes are targeted for expression. An experimental classification tool for ABC/GCB differentiation is available in Archer Analysis

Customize this panel by adding any of our functionally-tested designs. Assay Marketplace lets you browse our catalog of targeted AMP chemistry-based panels, modify an existing panel with additional targets, or create a new panel that fits your exact requirements.

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For Research Use Only. Not for use in diagnostic procedures.

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