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Archer™ VARIANTPlex™ Myeloid panel
Discover insights into myeloid-origin cancers with a targeted DNA panel
Detect single nucleotide variants, insertions, deletions, copy number variations, and internal tandem duplications with targeted NGS of 75 genes relevant for acute myeloid leukemia (AML), myeloproliferative neoplasms (MPN), myelodysplastic syndromes (MDS), and lymphoid malignancies research.
Detect confidently with Archer VARIANTPlex NGS Panels for DNA.
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Learn how the VARIANTPlex Myeloid panel can identify key genomic alterations for your research.
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Panel specifications
| Specifications | |
|---|---|
| Targeted genes | 75 |
| Genomic alterations | SNVs, Indels, CNVs, ITDs |
| Input nucleic acid required* | ≥10 ng |
| Recommended number of reads | 4 M |
| Hands-on time | <3.5 hours |
| Total library prep time | 1.5 days |
| Platform compatibility | Illumina® |
| Reagent format | Lyophilized or liquid |
| Supported sample types | Blood, bone marrow, fresh frozen, BMMC, PBMC |
*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50 ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200 ng is recommended.
Gene targets
ABL1
ANKRD26
ASXL1
ATRX
BCOR
BCORL1
BRAF
BTK
CALR
CBL
CBLB
CBLC
CCND2
CDC25C
CDKN2A
CEBPA
CSF3R
CUX1
CXCR4
DCK
DDX41
DHX15
DNMT3A
ETNK1
ETV6
EZH2
FBXW7
FLT3
GATA1
GATA2
GNAS
HRAS
IDH1
IDH2
IKZF1
JAK2
JAK3
KDM6A
KIT
KMT2A
KRAS
LUC7L2
MAP2K1
MPL
MYC
MYD88
NF1
NOTCH1
NPM1
NRAS
PDGFRA
PHF6
PPM1D
PTEN
PTPN11
RAD21
RBBP6
RPS14
RUNX1
SETBP1
SF3B1
SH2B3
SLC29A1
SMC1A
SMC3
SRSF2
STAG2
STAT3
TET2
TP53
U2AF1
U2AF2
WT1
XPO1
ZRSR2
Interested in adding a few genes to this panel?
Customize this NGS panel by adding any of our functionally-tested designs or create a new panel that fits your exact requirements with Assay Marketplace.
Benefits
- Detect confidently—A unique outlier detection algorithm leverages position-specific data to enable variant detection even at low allele frequencies, one of the many ways that Archer Archer Analysis empowers you to detect confidently.
- Less QNS—Anchored Multiplex PCR (AMP™) chemistry is designed for compatibility with a wide range of sample types, including low-input and degraded samples such as FFPE tissue.
- Achieve efficiency—Streamlined workflows for your lab are enabled by choice of reaction-sized lyophilized reagents or high-throughput liquid reagents, while parallel workflows across all Archer panels provide efficient genomic characterization.
- Customize content—Use Assay Marketplace to modify any panel to fit your lab’s needs or start from scratch to keep up with the pace of discovery.
Ready to start?
Talk with our technical sales team. Learn how the VARIANTPlex Myeloid panel can identify key genomic alterations for your research.
Request a consultationResources
Frequently asked questions
Can I analyze DNA and RNA simultaneously for comprehensive genomic profiling of blood cancers with Archer panels?
Yes, DNA and RNA from a single sample can be analyzed with VARIANTPlex™ and FUSIONPlex™ panels to provide a genomic profile of the blood cancer. For comprehensive genomic profiling, pair FUSIONPlex Pan Heme and VARIANTPlex Myeloid panels to interrogate 226 genes for fusions, splicing, exon-skipping, SNVs, indels, CNVs, ITDs and expression levels. Additionally, IMMUNOVerse™ assays can provide T- and B-cell clonality, MRD, and somatic hypermutation information relevant for heme malignancies. IMMUNOVerse panels have parallel workflows with VARIANTPlex and FUSIONPlex assays, allowing for comprehensive, streamlined blood cancer profiling.
What is the difference between Anchored Multiplex PCR (AMP™) and traditional priming methods for DNA NGS analysis?
As opposed to traditional priming methods, AMP chemistry enables tiling primers across both strands of DNA, optimally covering targeted regions for amplification and characterization of challenging, yet relevant alterations such as internal tandem duplications. Additionally, AMP chemistry uses molecular barcode adapters that bind to DNA fragments before amplification, allowing for efficient amplification of targets even from degraded or fragmented nucleic acid input, such as DNA from FFPE tissue and ctDNA from plasma.
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