FusionPlex Lung V2

Lung v2

The FusionPlex Lung v2 panel is a targeted next-generation sequencing (NGS) research product that expands on our well-adopted FusionPlex Lung panel to further drive discovery. The updated design covers newly published fusion breakpoints and isoforms of interest in key gene targets (including the NTRKs) and aligns with existing guidelines for non-small cell lung cancer research, including NCCN and ESMO (ESCAT). All gene content from the Lung v1 panel is covered in v2, including EGFR vIII and MET exon 14 skipping events along with prominent ALK, BRAF, FGFR, NRG1, NTRK, RET, and ROS1 fusions and select point mutations. The panel is powered by Anchored Multiplex PCR (AMP™)  target enrichment chemistry to detect fusions in a single sequencing product, even without prior knowledge of fusion partners or breakpoints.

  • Key Features
  • Specifications
  • Gene Targets
  • Additional Resources
Comprehensive Fusion Detection

AMP chemistry utilizes open-ended targeted amplification to identify gene fusions whether or not the fusion partner is known.

Splice Variants

Detect cancer-associated isoforms and alternative splicing events.

Low Input

Purpose-built for wide sample type compatibility, including low-input and degraded samples such as FFPE tissue.

Simple, Lyophilized Workflow

Easy-to-handle lyophilized reagents and a simple workflow to minimize errors and reduce turn-around time.

Properties

Specifications

Gene Targets
17
Input Nucleic Acid Required*
≤10ng
Unique Molecular On-Target %
>96%
Sample Types
Fresh frozen and FFPE
Hands-On Time
3.5 hour
Total Time
1.5 days
Platform
Illumina® and Ion Torrent™

*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200ng is recommended.

Product Information
Illumina
ION TORRENT™
Archer Analysis

ALK

BRAF

EGFR

ERBB2​

FGFR1

FGFR2

FGFR3

KRAS

MET

NRG1

NTRK1

NTRK2

NTRK3

NUTM1

PIK3CA

RET

ROS1

LEGEND

SNV/Indel
Fusion, splicing or exon-skipping

Need to modify your panel?

Add any of our wet lab-validated designs to this panel with Archer Assay Designer to build an assay that fits your exact requirements.

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For Research Use Only. Not for use in diagnostic procedures.

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For Research Use Only. Not for use in diagnostic procedures.