Core Solid Tumor
The VariantPlex Core Solid Tumor panel is a targeted next-generation sequencing (NGS) assay to detect copy number variations (CNVs), single-nucleotide variants (SNVs), insertions and deletions (indels), and microsatellite instability (MSI) status in 60 cancer-implicated genes, including non-small cell lung cancer, colorectal cancer, breast cancer, bladder cancer and melanoma. When used alongside an RNA-based FusionPlex® solid tumor panel, fusions, SNVs/indels, CNVs, MSI and RNA expression profiles can be analyzed from a single low-input FFPE sample.
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- Key Features
- Specifications
- Gene Targets
- Additional Resources
Orthogonal validation
Confirm relevant CNV and SNV/indel calls when using a companion FusionPlex solid tumor panel.
Customization
Create from scratch or modify any panel to keep up with the pace of discovery.
Low Input
Anchored Multiplex PCR (AMP™) chemistry was purpose-built for wide sample type compatibility, including low-input and degraded samples such as FFPE tissue.
Simple, Lyophilized Workflow
Easy-to-handle lyophilized reagents and a simple workflow to reduce turnaround time, as well as minimize errors and contamination risk.
Properties
Specifications
Gene Targets
60 + MSI
Input DNA Required*
≤10ng
Recommended # of reads
4.5M
Hands-On Time
<3.5 hours
Total Time
1.5 days
Platform
Illumina
Sample Types
FFPE, fresh frozen, cytology smear, FNA
*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200ng is recommended.
AKT1
ALK
APC
AR
ATRX
BRAF
CDK4
CDK6
CDKN2A
CTNNB1
DDR2
EGFR
ERBB2
ERBB3
ERBB4
ESR1
FBXW7
FGFR1
FGFR2
FGFR3
FOXL2
GNA11
GNAQ
GNAS
H3F3A
HIST1H3B
HRAS
IDH1
IDH2
JAK2
KIT
KRAS
MAP2K1
MAP2K2
MET
MTOR
MYC
NOTCH1
NOTCH2
NOTCH3
NOTCH4
NRAS
NTRK1
NTRK2
NTRK3
PDGFRA
PIK3CA
POLD1
POLE
PTEN
RAF1
RB1
RET
RICTOR
ROS1
SMAD4
SMO
TERT
TP53
VHL
LEGEND
SNV/Indel CNV
Product Information
Archer® Analysis
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