VariantPlex Expanded Solid Tumor

Expanded Solid Tumor

The VariantPlex Expanded Solid Tumor panel is a targeted next-generation sequencing (NGS) assay that simultaneously detects and characterizes copy number variations (CNVs), single-nucleotide variants (SNVs), insertions and deletions (indels), and microsatellite instability (MSI) status in 76 cancer-implicated genes, including non-small cell lung cancer, colorectal cancer, breast cancer, bladder cancer, pancreatic cancer, prostate cancer, cholangiocarcinoma and melanoma. Thoughtfully designed based on guidelines, literature, and customer input, this recently expanded panel offers sensitive variant detection even with low sample input.

  • Key Features
  • Specifications
  • Gene Targets
  • Additional Resources
Outlier detection

Invitae’s unique outlier detection algorithm leverages position-specific data to enable confident reporting at low allele frequencies.

Customization

Create from scratch or modify any panel to keep up with the pace of discovery.

Low Input

Anchored Multiplex PCR (AMP™) chemistry was purpose-built for wide sample type compatibility, including low-input and degraded samples such as FFPE tissue.

Simple, Lyophilized Workflow

Easy-to-handle lyophilized reagents and a simple workflow to reduce turnaround time, as well as minimize errors and contamination risk.

Properties

Specifications

Gene Targets
76 + MSI
Input DNA Required*
≤10ng
Recommended # of reads
6M
Hands-On Time
<3.5 hours
Total Time
1.5 days
Platform
Illumina
Sample Types
FFPE, fresh frozen, cytology smear, FNA

*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200ng is recommended.

AKT1

ALK

APC

AR

ATM

ATRX

BARD1

BRAF

BRCA1

BRCA2

BRIP1

CDK12

CDK4

CDK6

CDKN2A

CHEK1

CHEK2

CTNNB1

DDR2

EGFR

ERBB2

ERBB3

ERBB4

ESR1

FANCA

FANCL

FBXW7

FGFR1

FGFR2

FGFR3

FOXL2

GNA11

GNAQ

GNAS

H3F3A

HIST1H3B

HRAS

IDH1

IDH2

JAK2

KIT

KRAS

MAP2K1

MAP2K2

MET

MTOR

MYC

NOTCH1

NOTCH2

NOTCH3

NOTCH4

NRAS

NTRK1

NTRK2

NTRK3

PALB2

PDGFRA

PIK3CA

POLD1

POLE

PTEN

RAD51B

RAD51C

RAD51D

RAD54L

RAF1

RB1

RET

RICTOR

ROS1

SMAD4

SMO

STK11

TERT

TP53

VHL

LEGEND

   SNV/Indel
   CNV

Product Information
Illumina
  • AB0141 - 8 reaction kit
Archer® Analysis

Need to modify your panel?

Add any of our wet lab-validated designs to this panel with Archer Assay Designer to build an assay that fits your exact requirements.

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For Research Use Only. Not for use in diagnostic procedures.

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For Research Use Only. Not for use in diagnostic procedures.