VariantPlex Pan Solid Tumor

Pan Solid Tumor

The VariantPlex Pan Solid Tumor panel is a targeted next-generation sequencing (NGS) assay that simultaneously detects and characterizes copy number variations (CNVs), single-nucleotide variants (SNVs), insertions and deletions (indels), and microsatellite instability (MSI) status in 185 cancer-implicated genes, including non-small cell lung cancer, colorectal cancer, breast cancer, bladder cancer, gastric cancer, pancreatic cancer, prostate cancer, ovarian and uterine cancers, CNS cancers, cholangiocarcinoma and melanoma. Representing our most comprehensive DNA-based assay for solid tumor research, this newly expanded panel was thoughtfully designed based on guidelines, literature, and customer input, and offers sensitive variant detection even with low sample input. 

  • Key Features
  • Specifications
  • Gene Targets
  • Additional Resources
Outlier Detection

Invitae’s unique outlier detection algorithm leverages position-specific data to enable confident reporting at low allele frequencies.

Customization

Create from scratch or modify any panel to keep up with the pace of discovery.

Low Input

Anchored Multiplex PCR (AMP™) chemistry was purpose-built for wide sample type compatibility, including low-input and degraded samples such as FFPE tissue.

Simple, Lyophilized Workflow

Easy-to-handle lyophilized reagents and a simple workflow to minimize errors and reduce turnaround time.

Properties

Specifications

Gene Targets
185 + MSI
Input DNA Required*
≤10ng
Recommended # of reads
25M
Hands-On Time
<3.5 hours
Total Time
1.5 days
Platform
Illumina
Sample Types
FFPE, fresh frozen, cytology smear, FNA

*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200ng is recommended.

AKT1

BRAF

EGFR

ERBB2

FOXL2

GNA11

GNAQ

GNAS

HRAS

IDH1

IDH2

KIT

KRAS

MET

NRAS

PDGFRA

PIK3CA

RET

TERT

TP53

ALK

APC

AR

ATRX

CDK4

CDK6

CDKN2A

CTNNB1

DDR2

ERBB3

ERBB4

ESR1

FBXW7

FGFR1

FGFR2

H3F3A

HIST1H3B

JAK2

MAP2K1

MAP2K2

MTOR

MYC

NOTCH1

NOTCH2

NOTCH3

NOTCH4

NTKR1

NTKR12

NTKR13

POLD1

POLE

PTEN

RAF1

RB1

RICTOR

ROS1

SMAD4

SMO

VHL

ATM

BARD1

BRCA1

BRCA2

BRCA3

BRIP1

CDK12

CHEK1

CHEK2

FANCA

FANCL

PALB

RAD51B

RAD51C

RAD51D

RAD54L

STK11

ABL1

ACVR1

AKT2

AKT3

ARID1A

ARID1B

ARID2

ATR

AURKA

B2M

BAP1

BMPR1A

CCND1

CCND2

CCND3

CCNE1

CDH1

CDKN2B

CDH1

CIC

CSF1R

DAXX

DDX3X

DICER1

EIF1AX

EP300

EPCAM

ERCC1

ERCC2

EZH2

FANCI

FGF19

FGFR4

FH

FLCN

FLT1

FLT3

FLT4

FOXA1

FUBP1

H3F3B

HIST1H3C

HNF1A

JAK1

JAK3

KDM6A

KDR

KEAP1

KLF4

KMT2C

KMT2D

LZTR1

MAP3K1

MDM2

MDM4

MED12

MEN1

MLH1

MPL

MRE11A

MSH2

MSH3

MHS6

MUC16

MUTYH

MYCH

NBN

NF1

NF2

NKX2-1

NPM1

PBRM1

PIK3CB

PIK3R1

PLCB4

PMS2

PPP2R1A

PPP2R2A

RPRKD1

PTCH1

PTPN11

RAD50

RAD51

RHOA

RNF43

SDHA

SDHB

SDHC

SDHD

SETD2

SF3B1

SMAD2

SMARCA4

SMARCB1

SRC

SRSF2

STAG2

SUFU

TGFBR2

TP63

TRAF7

TSC1

TSC2

TSHR

U2AF1

XRCC2

XRCC3

XRCC3

LEGEND

   SNV/Indel
   CNV

Product Information
Illumina
  • AB0142 - 8 reaction kit
Customize this panel by adding any of our functionally-tested designs. Invitae’s Assay Marketplace lets you browse our catalog of targeted AMP chemistry-based panels, modify an existing panel with additional targets, or create a new panel that fits your exact requirements.

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For Research Use Only. Not for use in diagnostic procedures.

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For Research Use Only. Not for use in diagnostic procedures.