Solid Tumor Focus v2
The VariantPlex Solid Tumor Focus v2 panel is a targeted next-generation sequencing (NGS) assay that simultaneously detects and characterizes copy number variations (CNVs), single-nucleotide variants (SNVs), insertions and deletions (indels), and microsatellite instability (MSI) status in 20 cancer-implicated genes, including non-small cell lung cancer, colorectal cancer, breast cancer, and melanoma. Small enough to run on an Illumina® MiSeq, this DNA-based assay for solid tumor research offers sensitive variant detection even with low sample input.
Thank You
- Key Features
- Specifications
- Gene Targets
- Additional Resources
Outlier detection
Archer’s unique outlier detection algorithm leverages position-specific data to enable confident reporting at low allele frequencies.
Customization
Create from scratch or modify any panel to keep up with the pace of discovery.
Low Input
Anchored Multiplex PCR (AMP™) chemistry was purpose-built for wide sample type compatibility, including low-input and degraded samples such as FFPE tissue.
Simple, Lyophilized Workflow
Easy-to-handle lyophilized reagents and a simple workflow to reduce turnaround time, as well as minimize errors and contamination risk.
Properties
Specifications
Gene Targets
20 + MSI
Input DNA Required*
≤10ng
Recommended # of reads
1.5M
Hands-On Time
<3.5 hours
Total Time
1.5 days
Platform
Illumina
Sample Types
FFPE, fresh frozen, cytology smear, FNA
*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200ng is recommended.
AKT1
BRAF
EGFR
ERBB2
FOXL2
GNA11
GNAQ
GNAS
HRAS
IDH1
IDH2
KIT
KRAS
MET
NRAS
PDGFRA
PIK3CA
RET
TERT
TP53
LEGEND
SNV/Indel
CNV
Product Information
Archer® Analysis
Contact Us
