VariantPlex Solid Tumor Focus v2

Solid Tumor Focus v2

The VariantPlex Solid Tumor Focus v2 panel is a targeted next-generation sequencing (NGS) assay that simultaneously detects and characterizes copy number variations (CNVs), single-nucleotide variants (SNVs), insertions and deletions (indels), and microsatellite instability (MSI) status in 20 cancer-implicated genes, including non-small cell lung cancer, colorectal cancer, breast cancer, and melanoma. Small enough to run on an Illumina® MiSeq, this DNA-based assay for solid tumor research offers sensitive variant detection even with low sample input.

  • Key Features
  • Specifications
  • Gene Targets
  • Additional Resources
Outlier detection

Invitae’s unique outlier detection algorithm leverages position-specific data to enable confident reporting at low allele frequencies.

Customization

Create from scratch or modify any panel to keep up with the pace of discovery.

Low Input

Anchored Multiplex PCR (AMP™) chemistry was purpose-built for wide sample type compatibility, including low-input and degraded samples such as FFPE tissue.

Simple, Lyophilized Workflow

Easy-to-handle lyophilized reagents and a simple workflow to reduce turnaround time, as well as minimize errors and contamination risk.

Properties

Specifications

Gene Targets
20 + MSI
Input DNA Required*
≤10ng
Recommended # of reads
1.5M
Hands-On Time
<3.5 hours
Total Time
1.5 days
Platform
Illumina
Sample Types
FFPE, fresh frozen, cytology smear, FNA

*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200ng is recommended.

AKT1

BRAF

EGFR

ERBB2

FOXL2

GNA11

GNAQ

GNAS

HRAS

IDH1

IDH2

KIT

KRAS

MET

NRAS

PDGFRA

PIK3CA

RET

TERT

TP53

LEGEND

   SNV/Indel
   CNV

Product Information
Illumina
  • AB0139 - 8 reaction kit
Archer® Analysis

Need to modify your panel?

Add any of our wet lab-validated designs to this panel with Archer Assay Designer to build an assay that fits your exact requirements.

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For Research Use Only. Not for use in diagnostic procedures.

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For Research Use Only. Not for use in diagnostic procedures.