Solid Tumor Focus v2
The VariantPlex Solid Tumor Focus v2 panel is a targeted next-generation sequencing (NGS) assay that simultaneously detects and characterizes copy number variations (CNVs), single-nucleotide variants (SNVs), insertions and deletions (indels), and microsatellite instability (MSI) status in 20 cancer-implicated genes, including non-small cell lung cancer, colorectal cancer, breast cancer, and melanoma. Small enough to run on an Illumina® MiSeq, this DNA-based assay for solid tumor research offers sensitive variant detection even with low sample input.
Invitae’s unique outlier detection algorithm leverages position-specific data to enable confident reporting at low allele frequencies.
Create from scratch or modify any panel to keep up with the pace of discovery.
Anchored Multiplex PCR (AMP™) chemistry was purpose-built for wide sample type compatibility, including low-input and degraded samples such as FFPE tissue.
Simple, Lyophilized Workflow
Easy-to-handle lyophilized reagents and a simple workflow to reduce turnaround time, as well as minimize errors and contamination risk.
20 + MSI
Input DNA Required*
Recommended # of reads
FFPE, fresh frozen, cytology smear, FNA
*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200ng is recommended.