The minimum recommended input for the assay is 20ng of total nucleic acid. The amount of input material can be adjusted according to the quality of the RNA sample. When using less than 10ng of input material, the number of PCR cycles for the First and Second PCR may need to be increased. Note that reduced sample input may adversely affect read diversity. Please refer to the kit protocol for further instructions and information.
2477 55th Street, Suite 202
Boulder, CO 80301