Why does the read depth requirement listed on the website differ from that listed in the product insert?

The read depth requirements listed differ between the website and the product insert because the read depth will depend on the desired sensitivity of the end user. The website gives the recommended read depth to capture >95% of all possible SNV transitions with an allele fraction (AF) of 0.05, which is the most common AF desired. The read depth can be modified depending on your preferred sensitivity.

As shown in the left graph below, ~97% of all possible SNV transitions can be called at 0.05 AF when sequenced to a read depth of 1.5M with the VariantPlex® Core Myeloid kit, and thus 1.5M reads is listed on the website for VariantPlex Core Myeloid. If the read depth is doubled to 3M, ~97% of all possible SNV transitions can be called at half the allele fraction, 0.025 AF. For this reason, 3M reads are recommended in the product insert, as some users are interested in detecting sub-0.05 AF SNV transitions with this panel.

A similar trend is shown in the right graph below for the VariantPlex Myeloid kit. A read depth of 3M is recommended on the website as this will enable detection of >95% of SNV transitions at 0.05 AF. However, increasing the read depth to 4M reads will allow detection of >95% of sub-0.05 AF SNV transitions, so this is recommended in the product insert.

Sensitivity vs read depth for Core Myeloid
Sensitivity vs read depth for Myeloid


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For Research Use Only. Not for use in diagnostic procedures. For Research Use Only. Not for use in diagnostic procedures.