There are many commercially available controls containing T lymphocytes (for the TCR assays) or B lymphocytes (for BCR assays) that could be used for positive controls depending on experiment design. We recommend mixing Jurkat cell line RNA with peripheral blood leukocyte RNA to generate a positive control for the TCR assay, and the Raji cell line RNA mixed with peripheral blood leukocyte RNA to generate a positive control for the BCR assay. Both cell lines have a known clonotype that can be used in dilution experiments to assess low abundance clones.
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