Yes, the Immunoverse Kit can be used with FFPE samples. We have detected TCR and BCR clones within FFPE input samples using the Immunoverse kits. FFPE samples tend to be more degraded and produce fewer read-pairs across the CDR3 region. Complexity of the library and robust clonotype identification with Archer Analysis is driven by input quality, quantity, and B/T-cell content. Users should consider these factors in their experimental design -- especially when selecting RNA extraction procedures which affect the quality of the RNA. Please refer to the “Getting Started” section in the protocol for extraction recommendations.
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