No. Primers are designed in silico and ordered directly through Assay Designer. They are not tested in any way prior to shipping. They will arrive in an equimolar pool. For best results, it is recommended that you test the primer pool on DNA before use for fusion detection. Testing the primers on DNA will allow you to confirm that all primers are fully functional without the added complication of expression levels associated with target amplification of RNA. If you are interested in having ArcherDx functionally test your primers and balance your primer pools, contact technical support at firstname.lastname@example.org.
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