Technology
Elegant Chemistry. Purpose-Built.
Our Anchored Multiplex PCR (AMP™) chemistry is purpose-built to accurately identify both simple and complex genetic mutations by leveraging the power of bidirectional primers and molecular barcodes, or MBCs, from low nucleic acid input in tissue or blood. AMP chemistry is also flexible, so it can be used for applications in DNA, RNA, and ctDNA sequencing across most tumor types.
At the core of every panel is our AMP chemistry developed to create target-enriched libraries for next-generation sequencing (NGS).
Key Features
Molecular Barcodes (MBCS)
Adapters ligated to molecules before amplification carry MBCs that enable unique molecule counting and powerful error correction for confident mutation calling.
Adaptability
Targets can be added to panel to match the pace of discovery without negatively impacting panel performance.1
Strand-Specific Priming
Dual, independent coverage across target regions powers error correction and ensures that coverage is retained when one primer drops out due to primer-blocking SNVs.
Low Input
AMP chemistry was purpose-built for wide sample type compatibility, including low-input and degraded samples such as FFPE tissue.
Unique Start Sites
Open-ended amplification gives flexibility for novel fusion detection in RNA and strand-specific bidirectional coverage in DNA.
Application Flexibility
AMP chemistry can power new sequencing applications.
Efficient
AMP was designed to capture the mutated DNA, RNA, or ctDNA molecules even at low levels. It can reduce background noise and increases the sensitivity for detection of low-allelic fraction mutations, while retaining specificity.1
RNA Chemistry
DNA Chemistry
Footnotes:
- Zheng, Z., Zhelyazkova, B., Robinson, H., Iafrate, J. and Le, L. Anchored Multiplex PCR for Targeted Next Generation Sequencing. 2014