Technology

Elegant Chemistry. Purpose-Built.

Our Anchored Multiplex PCR (AMP™) chemistry is purpose-built to accurately identify both simple and complex genetic mutations by leveraging the power of bidirectional primers and molecular barcodes, or MBCs, from low nucleic acid input in tissue or blood. AMP chemistry is also flexible, so it can be used for applications in DNA, RNA, and ctDNA sequencing across most tumor types.

At the core of every Archer® panel is our AMP chemistry developed to create target-enriched libraries for next-generation sequencing (NGS).

Key Features

Molecular Barcodes (MBCS)

Adapters ligated to molecules before amplification carry MBCs that enable unique molecule counting and powerful error correction for confident mutation calling.

Adaptability

Targets can be added to panel to match the pace of discovery without negatively impacting panel performance.1

Strand-Specific Priming

Dual, independent coverage across target regions powers error correction and ensures that coverage is retained when one primer drops out due to primer-blocking SNVs.

Low Input

AMP chemistry was purpose-built for wide sample type compatibility, including low-input and degraded samples such as FFPE tissue.

Unique Start Sites

Open-ended amplification gives flexibility for novel fusion detection in RNA and strand-specific bidirectional coverage in DNA.

Application Flexibility

AMP chemistry can power new sequencing applications.

Efficient

AMP was designed to capture the mutated DNA, RNA, or ctDNA molecules even at low levels. It can reduce background noise and increases the sensitivity for detection of low-allelic fraction mutations, while retaining specificity.1

RNA Chemistry

DNA Chemistry

Footnotes:

  1. Zheng, Z., Zhelyazkova, B., Robinson, H., Iafrate, J. and Le, L. Anchored Multiplex PCR for Targeted Next Generation Sequencing. 2014