Complete Solid Tumor
The VariantPlex Complete Solid Tumor panel is a targeted next-generation sequencing (NGS) assay that simultaneously detects and characterizes copy number variations (CNVs), single-nucleotide variants (SNVs), insertions and deletions (indels), microsatellite instability (MSI) status, and tumor mutational burden (TMB) in 430 cancer-implicated genes, including non-small cell lung cancer, colorectal cancer, breast cancer, bladder cancer, gastric cancer, pancreatic cancer, prostate cancer, ovarian and uterine cancers, CNS cancers, cholangiocarcinoma and melanoma. Representing our most comprehensive DNA-based assay for solid tumor research, this newly expanded panel was thoughtfully designed based on guidelines, literature, and customer input, and offers analytically sensitive variant detection even with low sample input.
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- Key Features
- Specifications
- Gene Targets
- Additional Resources
Outlier Detection
Archer’s unique outlier detection algorithm leverages position-specific data to enable confident reporting at low allele frequencies.
Customization
Create from scratch or modify any panel to keep up with the pace of discovery.
Low Input
Anchored Multiplex PCR (AMP™) chemistry was purpose-built for wide sample type compatibility, including low-input and degraded samples such as FFPE tissue.
Simple, Lyophilized Workflow
Easy-to-handle lyophilized reagents and a simple workflow to minimize errors and reduce turnaround time.
Properties
Specifications
Gene Targets
430 + MSI + TMB
Input DNA Required*
≤10ng
Recommended # of reads
45M
Hands-On Time
<3.5 hours
Total Time
1.5 days
Platform
Illumina
Sample Types
FFPE, fresh frozen, cytology smear, FNA
*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200ng is recommended.
ABL1 | BLM | CHD4 | EPHB1 | FGFR4 | HOXB13 | KRT222 | MUC16 | PGR | PTPRC | SDHC | TCF7L2 |
ABL2 | BMPR1A | CHD8 | ERBB2 | FH | HRAS | LAMP1 | MUC6 | PHF6 | PTPRD | SDHD | TERT |
ACVR1 | BRAF | CHEK1 | ERBB3 | FLCN | HUWE1 | LATS1 | MUTYH | PIK3C2B | PTPRT | SETBP1 | TET1 |
ACVR2A | BRCA1 | CHEK2 | ERBB4 | FLNA | IDH1 | LATS2 | MYC | PIK3C2G | RAC1 | SETD2 | TET2 |
AKT1 | BRCA2 | CIC | ERCC1 | FLT1 | IDH2 | LEMD2 | MYCL | PIK3CA | RAD21 | SF3B1 | TFRC |
AKT2 | BRIP1 | COL5A1 | ERCC2 | FLT3 | IGF1R | LRP1B | MYCN | PIK3CB | RAD50 | SH2D1A | TGFBR1 |
AKT3 | BTG1 | CREBBP | ERCC4 | FLT4 | IKBKE | LZTR1 | MYD88 | PIK3CD | RAD51 | SLX4 | TGFBR2 |
ALK | BTG2 | CRKL | ERG | FOXA1 | IKZF1 | MACF1 | MYH9 | PIK3CG | RAD51B | SMAD2 | TGIF1 |
APC | BTK | CSDE1 | ERRFI1 | FOXA2 | IL6ST | MAP2K1 | NBN | PIK3R1 | RAD51C | SMAD3 | THRAP3 |
AR | CACNA1A | CSF1R | ESR1 | FOXL2 | IL7R | MAP2K2 | NCOR1 | PIK3R2 | RAD51D | SMAD4 | TLR4 |
ARAF | CARD11 | CSF3R | EWSR1 | FOXO1 | INPP4B | MAP2K4 | NF1 | PIK3R3 | RAD52 | SMARCA4 | TMSB4X |
ARHGAP35 | CASP8 | CTCF | EZH2 | FOXP1 | INPPL1 | MAP3K1 | NF2 | PIM1 | RAD54L | SMARCB1 | TNFAIP3 |
ARID1A | CBFB | CTLA4 | FAM175A | FOXQ1 | IRF2 | MAP3K13 | NFE2L2 | PLCB4 | RAF1 | SMC1A | TNFRSF14 |
ARID1B | CBL | CTNNA1 | FAM46C | FUBP1 | IRF4 | MAP3K4 | NFKBIA | PLCG1 | RARA | SMC3 | TP53 |
ARID2 | CCND1 | CTNNB1 | FAM46D | GATA1 | IRF6 | MAPK1 | NIPBL | PLXNB2 | RASA1 | SMO | TP63 |
ARID5B | CCND2 | CUL3 | FANCA | GATA2 | IRS2 | MAX | NKX2-1 | PMS1 | RB1 | SOCS1 | TRAF3 |
ASXL1 | CCND3 | CYSLTR2 | FANCC | GATA3 | JAK1 | MCL1 | NOTCH1 | PMS2 | RBM10 | SOX17 | TRAF7 |
ASXL2 | CCNE1 | DAXX | FANCD2 | GEN1 | JAK2 | MDM2 | NOTCH2 | POLD1 | RECQL4 | SOX2 | TSC1 |
ATM | CD274 | DDR2 | FANCE | GLI1 | JAK3 | MDM4 | NOTCH3 | POLE | REL | SOX9 | TSC2 |
ATR | CD70 | DDX3X | FANCF | GNA11 | JUN | MECOM | NOTCH4 | POLQ | RET | SPEN | TSHR |
ATRX | CD79A | DICER1 | FANCG | GNA13 | KANSL1 | MED12 | NPM1 | POLRMT | RHEB | SPOP | TXNIP |
AURKA | CD79B | DMD | FANCI | GNAQ | KDM5A | MEF2B | NRAS | PPARG | RHOA | SPTA1 | U2AF1 |
AURKB | CDC73 | DNMT3A | FANCL | GNAS | KDM5C | MEN1 | NSD1 | PPM1D | RICTOR | SPTAN1 | UNCX |
AXIN1 | CDH1 | DOT1L | FAT1 | GPS2 | KDM6A | MET | NTRK1 | PPP2R1A | RIT1 | SRC | USP9X |
AXIN2 | CDK12 | EEF1A1 | FBXW7 | GRIN2D | KDR | MGA | NTRK2 | PPP2R2A | RNF43 | SRSF2 | VHL |
AXL | CDK4 | EEF2 | FGF1 | GRM3 | KEAP1 | MGMT | NTRK3 | PPP6C | ROS1 | STAG1 | WT1 |
B2M | CDK6 | EGFR | FGF19 | H3F3A | KEL | MITF | NUP93 | PRDM1 | RPL5 | STAG2 | XPO1 |
BAP1 | CDK8 | EGR3 | FGF2 | H3F3B | KIF1A | MLH1 | PALB2 | PRKAR1A | RPS6KB1 | STAT3 | XRCC2 |
BARD1 | CDKN1A | EIF1AX | FGF3 | H3F3C | KIT | MLLT3 | PARP1 | PRKD1 | RPTOR | STK11 | XRCC3 |
BCL2 | CDKN1B | ELF3 | FGF4 | HGF | KLF4 | MPL | PAX5 | PRKDC | RRAS2 | SUFU | ZBTB20 |
BCL2L1 | CDKN2A | EP300 | FGF7 | HIST1H1C | KLF5 | MRE11A | PAX8 | PSIP1 | RUNX1 | SYK | ZFHX3 |
BCL2L11 | CDKN2B | EPAS1 | FGF8 | HIST1H1E | KMT2A | MSH2 | PBRM1 | PTCH1 | RUNX1T1 | TAF1 | ZMYM2 |
BCL6 | CDKN2C | EPCAM | FGF9 | HIST1H2BD | KMT2B | MSH3 | PDCD1 | PTEN | RXRA | TBL1XR1 | ZMYM3 |
BCOR | CEBPA | EPHA2 | FGFR1 | HIST1H3B | KMT2C | MSH6 | PDCD1LG2 | PTMA | SCAF4 | TBX3 | ZNF750 |
BCORL1 | CHD1 | EPHA3 | FGFR2 | HIST1H3C | KMT2D | MST1R | PDGFRA | PTPDC1 | SDHA | TCEB1 | |
BIRC3 | CHD3 | EPHA7 | FGFR3 | HNF1A | KRAS | MTOR | PDGFRB | PTPN11 | SDHB | TCF3 |