Pan Solid Tumor v2

The FusionPlex Pan Solid Tumor v2 panel is a targeted next-generation sequencing (NGS) research product that expands on the FusionPlex Solid Tumor panel to include key fusions and variants relevant across solid tumors and sarcomas in 137 genes. Representing our most comprehensive fusion coverage, the updated design includes key fusions and variants. This panel uses Anchored Multiplex PCR (AMP™)-based enrichment to detect all fusions associated with the genes in a single sequencing product, even without prior knowledge of fusion partners or breakpoints.

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Comprehensive Fusion Detection

AMP chemistry utilizes open-ended targeted amplification to identify gene fusions whether or not the fusion partner is known.

Splice Variants

Detect cancer-associated isoforms and alternative splicing events.

Low Input

Purpose-built for wide sample type compatibility, including low-input and degraded samples such as FFPE tissue.

Customization

Create from scratch or modify any panel to keep up with the pace of discovery.

Properties

Specifications

Gene Targets

137 Input Nucleic Acid Required*

≤10ng

Unique Molecular On-Target%

>96%

Hands-on Time

3.5 hours

Total Time

1.5 days

Platform

Illumina® and Ion Torrent™

Sample Types

Fresh frozen and FFPE

*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200ng is recommended.